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Assay Detail

CHEMBL3782567

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human GST tagged Tcf4/beta catenin interaction after 2 hrs using fluorescent AP Attophos substrate by ELISA analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

TCF4-CTNNB1 complex (CHEMBL3885541)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Target β-catenin/CD44/Nanog axis in colon cancer cells by certain N'-(2-oxoindolin-3-ylidene)-2-(benzyloxy)benzohydrazides.
Radwan AA, Al-Mohanna F, Alanazi FK, Manogaran PS, Al-Dhfyan A.
Bioorg Med Chem Lett (2016) 26 : 1664 -1670
PubMed 26944615 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 5.64 6.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3780110 1 6.19
CHEMBL578512 1 6.10
CHEMBL179197 1 5.62
CHEMBL261214 1 5.50
CHEMBL2397075 1 5.39
CHEMBL1080283 CERCOSPORIN 1 5.06

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3780110 IC50 = 640.0 nM 6.19
CHEMBL578512 IC50 = 800.0 nM 6.10
CHEMBL179197 IC50 = 2400.0 nM 5.62
CHEMBL261214 IC50 = 3200.0 nM 5.50
CHEMBL2397075 IC50 = 4100.0 nM 5.39
CHEMBL1080283 CERCOSPORIN IC50 = 8700.0 nM 5.06