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Assay Detail

CHEMBL3782899

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 24 hrs by Griess assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Diterpenoids from the Roots of Euphorbia fischeriana with Inhibitory Effects on Nitric Oxide Production.
Lee JW, Lee C, Jin Q, Jang H, Lee D, Lee HJ, Shin JW, Han SB, Hong JT, Kim Y, Lee MK, Hwang BY.
J Nat Prod (2016) 79 : 126 -131
PubMed 26702644 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 5.04 5.31

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL404387 JOLKINOLIDE B 1 5.31
CHEMBL3745928 1 5.25
CHEMBL3234204 1 5.13
CHEMBL3781335 1 5.03
CHEMBL6 INDOMETHACIN 4.0 1 4.90
CHEMBL3746430 1 4.90
CHEMBL225304 PIMAGEDINE 2.0 1 4.73
CHEMBL3234210 1 -
CHEMBL3234213 1 -
CHEMBL2165402 INGENOL 3.0 1 -
CHEMBL3781029 1 -
CHEMBL3746722 1 -
CHEMBL3780490 1 -
CHEMBL3747302 1 -
CHEMBL3746737 1 -
CHEMBL3746044 1 -
CHEMBL3779901 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL404387 JOLKINOLIDE B IC50 = 4900.0 nM 5.31
CHEMBL3745928 IC50 = 5600.0 nM 5.25
CHEMBL3234204 IC50 = 7400.0 nM 5.13
CHEMBL3781335 IC50 = 9400.0 nM 5.03
CHEMBL6 INDOMETHACIN IC50 = 12500.0 nM 4.90
CHEMBL3746430 IC50 = 12600.0 nM 4.90
CHEMBL225304 PIMAGEDINE IC50 = 18700.0 nM 4.73
CHEMBL3234210 IC50 > 20000.0 nM -
CHEMBL3234213 IC50 > 20000.0 nM -
CHEMBL2165402 INGENOL IC50 > 20000.0 nM -
CHEMBL3781029 IC50 > 20000.0 nM -
CHEMBL3746722 IC50 > 20000.0 nM -
CHEMBL3780490 IC50 > 20000.0 nM -
CHEMBL3747302 IC50 > 20000.0 nM -
CHEMBL3746737 IC50 > 20000.0 nM -
CHEMBL3746044 IC50 > 20000.0 nM -
CHEMBL3779901 IC50 > 20000.0 nM -