Assay Detail

CHEMBL3783328

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional

Cytotoxicity against human NCI-H23 cells after 48 hrs by sulforhodamine B assay

ChEMBL ↗

Free Research Context This assay will appear in recent viewed items on this browser. Open the demo workspace to preview project-only review queues.

Research home Demo workspace

Target

NCI-H23 (CHEMBL614997) ↗

Open DB target context

Use one target anchor to keep assay intent, target linkage, and tested compound review aligned.

Loading context

NCI-H23 (CHEMBL614997) is the working anchor for this assay review. Loading the live Open DB target snapshot…

Primary target NCI-H23

CHEMBL614997

Anchored from Assay target.

Protein identity NCI-H23

Disease frame Disease evidence pending

Open the live target snapshot or evidence card before reviewing assay metadata so target fit and disease framing stay reusable.

Review focus Open DB context pending

Open the live target snapshot before relying on this target frame across surface handoff.

Open target snapshot Open evidence card Search with this context

Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Functional
Organism	Homo sapiens
Cell Type	NCI-H23
Confidence	1 — Organism
Curated By	Autocuration

Target

NCI-H23 (CHEMBL614997) ↗

Type	CELL-LINE
Organism	Homo sapiens

Publication

Hydroxyurea derivatives of irofulven with improved antitumor efficacy.

Staake MD, Kashinatham A, McMorris TC, Estes LA, Kelner MJ.
Bioorg Med Chem Lett (2016) 26 : 1836 -1838
PubMed 26922141 · DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
IC50	2	7.45	7.80

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL3781819	LP-184	1.0	1	7.80
CHEMBL118218	IROFULVEN	3.0	1	7.10

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL3781819	LP-184	IC50	=	15.85	nM	7.80
CHEMBL118218	IROFULVEN	IC50	=	79.43	nM	7.10