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Assay Detail

CHEMBL3783356

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant His6 tagged human SIRT2 using 0.39 mM H2N-HK-[N(epsilon)-acetyl-lysine]-LM-COOH as substrate measured after 12 mins HPLC analysis in presence of 0.5 mM beta-NAD+
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

NAD-dependent protein deacetylase sirtuin-2 (CHEMBL4462)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Simple N(ε)-thioacetyl-lysine-containing cyclic peptides exhibiting highly potent sirtuin inhibition.
Huang Y, Liu J, Yan L, Zheng W.
Bioorg Med Chem Lett (2016) 26 : 1612 -1617
PubMed 26874402 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 7.01 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3781485 1 8.00
CHEMBL3781226 1 7.28
CHEMBL3780405 1 7.23
CHEMBL3780552 1 6.87
CHEMBL3781397 1 6.84
CHEMBL3781645 1 6.48
CHEMBL3781129 1 6.39

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3781485 IC50 = 10.1 nM 8.00
CHEMBL3781226 IC50 = 53.1 nM 7.28
CHEMBL3780405 IC50 = 58.9 nM 7.23
CHEMBL3780552 IC50 = 134.0 nM 6.87
CHEMBL3781397 IC50 = 143.6 nM 6.84
CHEMBL3781645 IC50 = 332.0 nM 6.48
CHEMBL3781129 IC50 = 404.3 nM 6.39