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Assay Detail

CHEMBL3783933

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human CYP17A1 expressed in HEK293 cell microsomes using [3H]-pregnenolone as substrate incubated for 45 mins by scintillation proximity assay in presence of NADPH
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Steroid 17-alpha-hydroxylase/17,20 lyase (CHEMBL3522)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of the Selective CYP17A1 Lyase Inhibitor BMS-351 for the Treatment of Prostate Cancer.
Huang A, Jayaraman L, Fura A, Vite GD, Trainor GL, Gottardis MM, Spires TE, Spires VM, Rizzo CA, Obermeier MT, Elzinga PA, Todderud G, Fan Y, Newitt JA, Beyer SM, Zhu Y, Warrack BM, Goodenough AK, Tebben AJ, Doweyko AM, Gold DL, Balog A.
ACS Med Chem Lett (2016) 7 : 40 -45
PubMed 26819663 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 8.21 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3782020 1 8.70
CHEMBL3780658 1 8.52
CHEMBL3780266 1 8.40
CHEMBL3780743 1 8.40
CHEMBL3781112 1 8.30
CHEMBL3780226 1 8.30
CHEMBL3780048 1 8.30
CHEMBL2147034 1 8.22
CHEMBL3781487 1 8.10
CHEMBL3781910 1 8.10
CHEMBL2147041 1 7.72
CHEMBL2147035 1 7.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3782020 IC50 = 2.0 nM 8.70
CHEMBL3780658 IC50 = 3.0 nM 8.52
CHEMBL3780266 IC50 = 4.0 nM 8.40
CHEMBL3780743 IC50 = 4.0 nM 8.40
CHEMBL3781112 IC50 = 5.0 nM 8.30
CHEMBL3780226 IC50 = 5.0 nM 8.30
CHEMBL3780048 IC50 = 5.0 nM 8.30
CHEMBL2147034 IC50 = 6.0 nM 8.22
CHEMBL3781487 IC50 = 8.0 nM 8.10
CHEMBL3781910 IC50 = 8.0 nM 8.10
CHEMBL2147041 IC50 = 19.0 nM 7.72
CHEMBL2147035 IC50 = 37.0 nM 7.43