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Assay Detail

CHEMBL3801484

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Functional
Agonist activity at human beta2 receptor expressed in HEK293 cells cotransfected with Gqs5 protein assessed as [3H]IP3 accumulation after 120 mins by scintillation counting assay in presence of myo-[3H]-inositol
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-2 adrenergic receptor (CHEMBL210)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-guided development of dual β2 adrenergic/dopamine D2 receptor agonists.
Weichert D, Stanek M, Hübner H, Gmeiner P.
Bioorg Med Chem (2016) 24 : 2641 -2653
PubMed 27132867 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 6.33 8.21

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3800400 1 8.21
CHEMBL3798748 1 8.15
CHEMBL434 ISOPROTERENOL 4.0 1 7.06
CHEMBL3797876 1 6.92
CHEMBL3797758 1 6.14
CHEMBL3800468 1 6.08
CHEMBL3797205 1 6.00
CHEMBL3799593 1 5.85
CHEMBL3799427 1 5.58
CHEMBL3800303 1 5.29
CHEMBL3800636 1 4.33
CHEMBL240773 QUINPIROLE 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3800400 EC50 = 6.2 nM 8.21
CHEMBL3798748 EC50 = 7.0 nM 8.15
CHEMBL434 ISOPROTERENOL EC50 = 87.0 nM 7.06
CHEMBL3797876 EC50 = 120.0 nM 6.92
CHEMBL3797758 EC50 = 720.0 nM 6.14
CHEMBL3800468 EC50 = 840.0 nM 6.08
CHEMBL3797205 EC50 = 1000.0 nM 6.00
CHEMBL3799593 EC50 = 1400.0 nM 5.85
CHEMBL3799427 EC50 = 2600.0 nM 5.58
CHEMBL3800303 EC50 = 5100.0 nM 5.29
CHEMBL3800636 EC50 = 47000.0 nM 4.33
CHEMBL240773 QUINPIROLE EC50 - -