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Assay Detail

CHEMBL3813261

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Binding
Inhibition of Escherichia coli GroEL/GroES using Escherichia coli GroEL expressed in Escherichia coli DH5alpha cells and Escherichia coli GroES expressed in Escherichia coli BL21(DE3) cells assessed as reduction in native refolded rhodanese-mediated thiocyanate production by micro plate reader analysis
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

GroEL/GroES (CHEMBL4106139)
Type PROTEIN COMPLEX
Organism Escherichia coli

Publication

GroEL/ES inhibitors as potential antibiotics.
Abdeen S, Salim N, Mammadova N, Summers CM, Frankson R, Ambrose AJ, Anderson GG, Schultz PG, Horwich AL, Chapman E, Johnson SM.
Bioorg Med Chem Lett (2016) 26 : 3127 -3134
PubMed 27184767 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 5.23 6.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3115913 1 6.60
CHEMBL1411862 1 5.85
CHEMBL1557710 1 5.68
CHEMBL1713063 1 5.60
CHEMBL3115927 1 5.28
CHEMBL3115920 1 5.09
CHEMBL3116086 1 5.00
CHEMBL3115926 1 4.92
CHEMBL3115914 1 4.92
CHEMBL3115911 1 4.85
CHEMBL3112623 1 4.64
CHEMBL3115929 1 4.28
CHEMBL3115921 1 -
CHEMBL3115919 1 -
CHEMBL3115924 1 -
CHEMBL3115912 1 -
CHEMBL1632145 1 -
CHEMBL3115928 1 -
CHEMBL3115908 1 -
CHEMBL3116087 1 -
CHEMBL3116088 1 -
CHEMBL3116089 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3115913 IC50 = 250.0 nM 6.60
CHEMBL1411862 IC50 = 1400.0 nM 5.85
CHEMBL1557710 IC50 = 2100.0 nM 5.68
CHEMBL1713063 IC50 = 2500.0 nM 5.60
CHEMBL3115927 IC50 = 5300.0 nM 5.28
CHEMBL3115920 IC50 = 8100.0 nM 5.09
CHEMBL3116086 IC50 = 10000.0 nM 5.00
CHEMBL3115926 IC50 = 12000.0 nM 4.92
CHEMBL3115914 IC50 = 12000.0 nM 4.92
CHEMBL3115911 IC50 = 14000.0 nM 4.85
CHEMBL3112623 IC50 = 23000.0 nM 4.64
CHEMBL3115929 IC50 = 52000.0 nM 4.28
CHEMBL3115921 IC50 > 100000.0 nM -
CHEMBL3115919 IC50 > 100000.0 nM -
CHEMBL3115924 IC50 > 100000.0 nM -
CHEMBL3115912 IC50 > 100000.0 nM -
CHEMBL1632145 IC50 > 100000.0 nM -
CHEMBL3115928 IC50 > 100000.0 nM -
CHEMBL3115908 IC50 > 100000.0 nM -
CHEMBL3116087 IC50 > 100000.0 nM -
CHEMBL3116088 IC50 > 100000.0 nM -
CHEMBL3116089 IC50 > 100000.0 nM -