Assay Detail
Binding
CHEMBL3813356
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of EGFR-TK in human A431 cell lysate assessed as reduction in EGF stimulated kinase activity after 60 mins using biotinylated peptide substrate by ELISA
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | A-431 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Combined inhibition of the EGFR/AKT pathways by a novel conjugate of quinazoline with isothiocyanate.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 5.94 | 7.44 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL285063 | — | — | 1 | 7.44 |
| CHEMBL3809045 | — | — | 1 | 6.06 |
| CHEMBL52765 | — | — | 1 | 5.21 |
| CHEMBL48802 | SULFORAPHANE | 3.0 | 1 | 5.05 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL285063 | — | IC50 | = | 36.0 | nM | 7.44 |
| CHEMBL3809045 | — | IC50 | = | 880.0 | nM | 6.06 |
| CHEMBL52765 | — | IC50 | = | 6100.0 | nM | 5.21 |
| CHEMBL48802 | SULFORAPHANE | IC50 | = | 9000.0 | nM | 5.05 |