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Assay Detail

CHEMBL3815445

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR L858R/T790M double mutant (unknown origin) expressed in mouse BaF/3 cells assessed as cell growth inhibition after 72 hrs by MTS assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Toward discovery of mutant EGFR inhibitors; Design, synthesis and in vitro biological evaluation of potent 4-arylamino-6-ureido and thioureido-quinazoline derivatives.
Mowafy S, Galanis A, Doctor ZM, Paranal RM, Lasheen DS, Farag NA, Jänne PA, Abouzid KA.
Bioorg Med Chem (2016) 24 : 3501 -3512
PubMed 27288180 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.71 5.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3814940 1 5.75
CHEMBL3814021 1 5.69
CHEMBL3814846 1 5.68
CHEMBL939 GEFITINIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3814940 IC50 = 1760.0 nM 5.75
CHEMBL3814021 IC50 = 2030.0 nM 5.69
CHEMBL3814846 IC50 = 2100.0 nM 5.68
CHEMBL939 GEFITINIB IC50 > 10000.0 nM -