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Assay Detail

CHEMBL3816166

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant PI3Kalpha (unknown origin) using dioctanoylglycerol-PIP2 as substrate incubated for 30 mins in presence of TAMRA-PIP3 by fluorescence polarization assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of Clinical Development Candidate GDC-0084, a Brain Penetrant Inhibitor of PI3K and mTOR.
Heffron TP, Ndubaku CO, Salphati L, Alicke B, Cheong J, Drobnick J, Edgar K, Gould SE, Lee LB, Lesnick JD, Lewis C, Nonomiya J, Pang J, Plise EG, Sideris S, Wallin J, Wang L, Zhang X, Olivero AG.
ACS Med Chem Lett (2016) 7 : 351 -356
PubMed 27096040 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 8.51 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2178606 1 9.00
CHEMBL3813842 PAXALISIB 2.0 1 8.70
CHEMBL3814414 1 8.70
CHEMBL2178608 1 8.70
CHEMBL3814925 1 8.52
CHEMBL3814544 1 8.52
CHEMBL3813906 1 8.52
CHEMBL3815084 1 8.30
CHEMBL3814365 1 8.30
CHEMBL1922094 APITOLISIB 2.0 1 8.30
CHEMBL3814933 1 8.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2178606 Ki = 1.0 nM 9.00
CHEMBL3813842 PAXALISIB Ki = 2.0 nM 8.70
CHEMBL3814414 Ki = 2.0 nM 8.70
CHEMBL2178608 Ki = 2.0 nM 8.70
CHEMBL3814925 Ki = 3.0 nM 8.52
CHEMBL3814544 Ki = 3.0 nM 8.52
CHEMBL3813906 Ki = 3.0 nM 8.52
CHEMBL3815084 Ki = 5.0 nM 8.30
CHEMBL3814365 Ki = 5.0 nM 8.30
CHEMBL1922094 APITOLISIB Ki = 5.0 nM 8.30
CHEMBL3814933 Ki = 10.0 nM 8.00