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Assay Detail

CHEMBL3819964

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human POP expressed in Escherichia coli BL21 pre-incubated for 30 mins before ZGP-pNA substrate addition
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prolyl endopeptidase (CHEMBL3202)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

3-Oxo-hexahydro-1H-isoindole-4-carboxylic Acid as a Drug Chiral Bicyclic Scaffold: Structure-Based Design and Preparation of Conformationally Constrained Covalent and Noncovalent Prolyl Oligopeptidase Inhibitors.
Mariaule G, De Cesco S, Airaghi F, Kurian J, Schiavini P, Rocheleau S, Huskić I, Auclair K, Mittermaier A, Moitessier N.
J Med Chem (2016) 59 : 4221 -4234
PubMed 26619267 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 21 6.02 9.02

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL189620 1 9.02
CHEMBL3817958 1 9.00
CHEMBL3818013 1 8.00
CHEMBL2159748 1 7.64
CHEMBL3818020 1 7.49
CHEMBL3819136 1 7.34
CHEMBL3819096 1 6.00
CHEMBL3818815 1 5.82
CHEMBL3818902 1 5.68
CHEMBL3819346 1 5.40
CHEMBL3819362 1 5.12
CHEMBL3819408 1 4.92
CHEMBL3818446 1 4.21
CHEMBL3818472 1 4.20
CHEMBL3819072 1 4.19
CHEMBL3818541 1 4.17
CHEMBL3818999 1 4.10
CHEMBL3818703 1 -
CHEMBL3819297 1 -
CHEMBL3819233 1 -
CHEMBL3818697 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL189620 Ki = 0.95 nM 9.02
CHEMBL3817958 Ki = 1.0 nM 9.00
CHEMBL3818013 Ki = 10.0 nM 8.00
CHEMBL2159748 Ki = 23.0 nM 7.64
CHEMBL3818020 Ki = 32.5 nM 7.49
CHEMBL3819136 Ki = 46.0 nM 7.34
CHEMBL3819096 Ki = 1000.0 nM 6.00
CHEMBL3818815 Ki = 1500.0 nM 5.82
CHEMBL3818902 Ki = 2100.0 nM 5.68
CHEMBL3819346 Ki = 4000.0 nM 5.40
CHEMBL3819362 Ki = 7500.0 nM 5.12
CHEMBL3819408 Ki = 12000.0 nM 4.92
CHEMBL3818446 Ki = 62000.0 nM 4.21
CHEMBL3818472 Ki = 62500.0 nM 4.20
CHEMBL3819072 Ki = 65000.0 nM 4.19
CHEMBL3818541 Ki = 67000.0 nM 4.17
CHEMBL3818999 Ki = 80000.0 nM 4.10
CHEMBL3818703 Ki > 150000.0 nM -
CHEMBL3819297 Ki > 150000.0 nM -
CHEMBL3819233 Ki > 150000.0 nM -
CHEMBL3818697 Ki > 150000.0 nM -