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Assay Detail

CHEMBL3820514

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length recombinant human N-terminal GST-tagged TTK using biotinylated PWDPDDADITEILG as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by TR-FRET assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dual specificity protein kinase TTK (CHEMBL3983)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 4-(4-aminopyrazolo[1,5-a][1,3,5]triazin-8-yl)benzamides as novel, highly potent and selective, orally bioavailable inhibitors of Tyrosine Threonine Kinase, TTK.
Laufer R, Li SW, Liu Y, Ng G, Lang Y, Feher M, Brokx R, Beletskaya I, Hodgson R, Mao G, Plotnikova O, Awrey DE, Mason JM, Wei X, Lin DC, Che Y, Kiarash R, Madeira B, Fletcher GC, Mak TW, Bray MR, Pauls HW.
Bioorg Med Chem Lett (2016) 26 : 3562 -3566
PubMed 27335255 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 8.78 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3422104 1 9.00
CHEMBL3819182 1 8.55

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3422104 IC50 = 1.0 nM 9.00
CHEMBL3819182 IC50 = 2.8 nM 8.55