Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3820977

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at human C-terminal eYFP-tagged FFA1 receptor expressed in HEK-293 cells assessed as beta-arrestin2 recruitment incubated for 5 mins by BRET assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Free fatty acid receptor 1 (CHEMBL4422)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development and Characterization of a Potent Free Fatty Acid Receptor 1 (FFA1) Fluorescent Tracer.
Christiansen E, Hudson BD, Hansen AH, Milligan G, Ulven T.
J Med Chem (2016) 59 : 4849 -4858
PubMed 27074625 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 11 7.17 8.03

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1829174 FASIGLIFAM 3.0 1 8.03
CHEMBL1773261 1 7.95
CHEMBL2164242 1 7.85
CHEMBL2386353 1 7.73
CHEMBL207881 GW9508 1 7.55
CHEMBL604126 1 7.26
CHEMBL2315528 1 7.21
CHEMBL449588 1 7.17
CHEMBL1829173 1 6.81
CHEMBL1688452 BENZYLOXYPHENYLPROPANOIC ACID 1 6.26
CHEMBL108766 DODECANOATE 1 5.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1829174 FASIGLIFAM EC50 = 9.333 nM 8.03
CHEMBL1773261 EC50 = 11.22 nM 7.95
CHEMBL2164242 EC50 = 14.13 nM 7.85
CHEMBL2386353 EC50 = 18.62 nM 7.73
CHEMBL207881 GW9508 EC50 = 28.18 nM 7.55
CHEMBL604126 EC50 = 54.95 nM 7.26
CHEMBL2315528 EC50 = 61.66 nM 7.21
CHEMBL449588 EC50 = 67.61 nM 7.17
CHEMBL1829173 EC50 = 154.88 nM 6.81
CHEMBL1688452 BENZYLOXYPHENYLPROPANOIC ACID EC50 = 549.54 nM 6.26
CHEMBL108766 DODECANOATE EC50 = 7943.28 nM 5.10