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Assay Detail

CHEMBL3821775

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human serum butyrylcholinesterase preincubated for 5 mins followed by addition of S-butyrylthiocholine iodide as substrate measured after 60 to 120 mins by Ellmans method
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cholinesterase (CHEMBL1914)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and evaluation of donepezilferulic acid hybrids as multi-target-directed ligands against Alzheimer's disease
Xu W, Wang X, Wang Z, Wu J, Li F, Wang J, Kong L
Medchemcomm (2016) 7 : 990 -998
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.82 6.61

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3819383 1 6.61
CHEMBL3818250 1 6.54
CHEMBL3819083 1 6.17
CHEMBL3819320 1 6.11
CHEMBL3817981 1 6.00
CHEMBL3818089 1 5.95
CHEMBL3819231 1 5.91
CHEMBL3819548 1 5.85
CHEMBL3818512 1 5.84
CHEMBL3818990 1 5.68
CHEMBL3818689 1 5.59
CHEMBL3818086 1 5.49
CHEMBL3818782 1 5.37
CHEMBL3818332 1 5.12
CHEMBL502 DONEPEZIL 4.0 1 5.08
CHEMBL3818992 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3819383 IC50 = 245.0 nM 6.61
CHEMBL3818250 IC50 = 286.0 nM 6.54
CHEMBL3819083 IC50 = 669.0 nM 6.17
CHEMBL3819320 IC50 = 784.0 nM 6.11
CHEMBL3817981 IC50 = 988.0 nM 6.00
CHEMBL3818089 IC50 = 1120.0 nM 5.95
CHEMBL3819231 IC50 = 1220.0 nM 5.91
CHEMBL3819548 IC50 = 1410.0 nM 5.85
CHEMBL3818512 IC50 = 1440.0 nM 5.84
CHEMBL3818990 IC50 = 2070.0 nM 5.68
CHEMBL3818689 IC50 = 2540.0 nM 5.59
CHEMBL3818086 IC50 = 3240.0 nM 5.49
CHEMBL3818782 IC50 = 4230.0 nM 5.37
CHEMBL3818332 IC50 = 7510.0 nM 5.12
CHEMBL502 DONEPEZIL IC50 = 8280.0 nM 5.08
CHEMBL3818992 IC50 = 145100.0 nM -