Assay Detail
Binding
CHEMBL3824698
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Activation of VDR in human Caco2 cells expressing lentiviral VDRE-luciferase vector assessed as VDRE-mediated transcriptional activity measured after 24 hrs by luciferase transcriptional reporter assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Caco-2 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Synthesis and Biological Evaluation of Vitamin D3 Metabolite 20S,23S-Dihydroxyvitamin D3 and Its 23R Epimer.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 5 | 6.62 | 6.84 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL333950 | MAXACALCITOL | 3.0 | 1 | 6.84 |
| CHEMBL3822832 | — | — | 1 | 6.76 |
| CHEMBL846 | CALCITRIOL | 4.0 | 1 | 6.65 |
| CHEMBL3822994 | — | — | 1 | 6.62 |
| CHEMBL3823776 | — | — | 1 | 6.25 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL333950 | MAXACALCITOL | EC50 | = | 144.5 | nM | 6.84 |
| CHEMBL3822832 | — | EC50 | = | 171.7 | nM | 6.76 |
| CHEMBL846 | CALCITRIOL | EC50 | = | 223.4 | nM | 6.65 |
| CHEMBL3822994 | — | EC50 | = | 241.4 | nM | 6.62 |
| CHEMBL3823776 | — | EC50 | = | 562.0 | nM | 6.25 |