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Assay Detail

CHEMBL3825019

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-tagged rat recombinant DYRK1A expressed in Escherichia coli using myelin basic protein as substrate incubated for 30 mins in presence of [gamma-33 ATP] by liquid scintillation counting analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dual specificity tyrosine-phosphorylation-regulated kinase 1A (CHEMBL5508)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Discovery of pyrido[3,4-g]quinazoline derivatives as CMGC family protein kinase inhibitors: Design, synthesis, inhibitory potency and X-ray co-crystal structure.
Esvan YJ, Zeinyeh W, Boibessot T, Nauton L, Théry V, Knapp S, Chaikuad A, Loaëc N, Meijer L, Anizon F, Giraud F, Moreau P.
Eur J Med Chem (2016) 118 : 170 -177
PubMed 27128181 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 6.84 7.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3823483 1 7.55
CHEMBL3823505 1 6.75
CHEMBL3823532 1 6.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3823483 IC50 = 28.0 nM 7.55
CHEMBL3823505 IC50 = 180.0 nM 6.75
CHEMBL3823532 IC50 = 620.0 nM 6.21