Assay Detail
Binding
CHEMBL3829040
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human recombinant HDAC10 expressed in baculovirus infected insect Sf9 cells using Ac-peptide-AMC as substrate assessed as release of AMC preincubated for 15 mins followed by substrate addition measured after 1 hr by fluorescence assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Development of Purine-Based Hydroxamic Acid Derivatives: Potent Histone Deacetylase Inhibitors with Marked in Vitro and in Vivo Antitumor Activities.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 7 | 8.43 | 9.07 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3827517 | — | — | 1 | 9.07 |
| CHEMBL3827814 | — | — | 1 | 8.55 |
| CHEMBL3827894 | — | — | 1 | 8.55 |
| CHEMBL3622533 | FIMEPINOSTAT | 2.0 | 1 | 8.55 |
| CHEMBL483254 | PANOBINOSTAT | 4.0 | 1 | 8.35 |
| CHEMBL3828518 | — | — | 1 | 8.16 |
| CHEMBL3827281 | — | — | 1 | 7.76 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3827517 | — | IC50 | = | 0.86 | nM | 9.07 |
| CHEMBL3827814 | — | IC50 | = | 2.8 | nM | 8.55 |
| CHEMBL3827894 | — | IC50 | = | 2.83 | nM | 8.55 |
| CHEMBL3622533 | FIMEPINOSTAT | IC50 | = | 2.8 | nM | 8.55 |
| CHEMBL483254 | PANOBINOSTAT | IC50 | = | 4.45 | nM | 8.35 |
| CHEMBL3828518 | — | IC50 | = | 6.85 | nM | 8.16 |
| CHEMBL3827281 | — | IC50 | = | 17.55 | nM | 7.76 |