Assay Detail
Binding
CHEMBL3829094
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Inhibition of recombinant Escherichia coli DHFR expressed in Escherichia coli BL21(DE3) cells assessed as reduction in NADPH oxidation using dihydrofolate as substrate preincubated for 5 mins followed by substrate addition
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Charged Nonclassical Antifolates with Activity Against Gram-Positive and Gram-Negative Pathogens.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 9 | 8.76 | 9.66 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL22 | TRIMETHOPRIM | 4.0 | 1 | 9.66 |
| CHEMBL3828485 | — | — | 1 | 9.04 |
| CHEMBL3827532 | — | — | 1 | 9.01 |
| CHEMBL3828581 | — | — | 1 | 8.74 |
| CHEMBL3827326 | — | — | 1 | 8.71 |
| CHEMBL3827086 | — | — | 1 | 8.67 |
| CHEMBL3827760 | — | — | 1 | 8.50 |
| CHEMBL3828291 | — | — | 1 | 8.26 |
| CHEMBL3828724 | — | — | 1 | 8.24 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL22 | TRIMETHOPRIM | Ki | = | 0.22 | nM | 9.66 |
| CHEMBL3828485 | — | Ki | = | 0.91 | nM | 9.04 |
| CHEMBL3827532 | — | Ki | = | 0.98 | nM | 9.01 |
| CHEMBL3828581 | — | Ki | = | 1.806 | nM | 8.74 |
| CHEMBL3827326 | — | Ki | = | 1.93 | nM | 8.71 |
| CHEMBL3827086 | — | Ki | = | 2.12 | nM | 8.67 |
| CHEMBL3827760 | — | Ki | = | 3.14 | nM | 8.50 |
| CHEMBL3828291 | — | Ki | = | 5.52 | nM | 8.26 |
| CHEMBL3828724 | — | Ki | = | 5.72 | nM | 8.24 |