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Assay Detail

CHEMBL3829624

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human C-terminal His-tagged ATX expressed in HEK293E cells using lysophosphatidylcholine as substrate preincubated with enzyme followed by substrate addition measured after 1 hr by AmplexRed dye based fluorescence assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293E
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Autotaxin (CHEMBL3691)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of Autotaxin Inhibitors: An Overview of the Patent and Primary Literature.
Castagna D, Budd DC, Macdonald SJ, Jamieson C, Watson AJ.
J Med Chem (2016) 59 : 5604 -5621
PubMed 26745766 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 9.00 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3828650 1 9.00
CHEMBL3827513 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3828650 IC50 = 1.0 nM 9.00
CHEMBL3827513 IC50 < 1.7 nM -