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Assay Detail

CHEMBL3830587

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Alexa Fluor 647-tagged I-BET762 binding to recombinant truncated 6H-Thr BRD4 BD1 Y97A mutant (1 to 477 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells after 30 mins by TR-FRET assay
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type BL21-DE3
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

GSK6853, a Chemical Probe for Inhibition of the BRPF1 Bromodomain.
Bamborough P, Barnett HA, Becher I, Bird MJ, Chung CW, Craggs PD, Demont EH, Diallo H, Fallon DJ, Gordon LJ, Grandi P, Hobbs CI, Hooper-Greenhill E, Jones EJ, Law RP, Le Gall A, Lugo D, Michon AM, Mitchell DJ, Prinjha RK, Sheppard RJ, Watson AJ, Watson RJ.
ACS Med Chem Lett (2016) 7 : 552 -557
PubMed 27326325 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 4.80 5.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3827767 1 5.10
CHEMBL3828194 1 5.00
CHEMBL3356145 1 5.00
CHEMBL3828311 1 5.00
CHEMBL3827114 1 5.00
CHEMBL3828008 1 4.90
CHEMBL3827280 1 4.70
CHEMBL3828191 1 4.70
CHEMBL3827276 1 4.60
CHEMBL3827927 1 4.40
CHEMBL3828061 1 4.40
CHEMBL3827629 1 -
CHEMBL3828186 1 -
CHEMBL3827941 1 -
CHEMBL3828546 1 -
CHEMBL3827710 1 -
CHEMBL3828299 1 -
CHEMBL3356143 1 -
CHEMBL3827158 1 -
CHEMBL3828504 1 -
CHEMBL3827434 1 -
CHEMBL3828357 1 -
CHEMBL3828426 1 -
CHEMBL3828684 1 -
CHEMBL3828026 1 -
CHEMBL3828354 1 -
CHEMBL3356146 1 -
CHEMBL3827560 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3827767 IC50 = 7943.28 nM 5.10
CHEMBL3828194 IC50 = 10000.0 nM 5.00
CHEMBL3356145 IC50 = 10000.0 nM 5.00
CHEMBL3828311 IC50 = 10000.0 nM 5.00
CHEMBL3827114 IC50 = 10000.0 nM 5.00
CHEMBL3828008 IC50 = 12589.25 nM 4.90
CHEMBL3827280 IC50 = 19952.62 nM 4.70
CHEMBL3828191 IC50 = 19952.62 nM 4.70
CHEMBL3827276 IC50 = 25118.86 nM 4.60
CHEMBL3827927 IC50 = 39810.72 nM 4.40
CHEMBL3828061 IC50 = 39810.72 nM 4.40
CHEMBL3827629 IC50 > 50118.72 nM -
CHEMBL3828186 IC50 > 50118.72 nM -
CHEMBL3827941 IC50 > 50118.72 nM -
CHEMBL3828546 IC50 > 50118.72 nM -
CHEMBL3827710 IC50 > 50118.72 nM -
CHEMBL3828299 IC50 > 50118.72 nM -
CHEMBL3356143 IC50 > 50118.72 nM -
CHEMBL3827158 IC50 > 50118.72 nM -
CHEMBL3828504 IC50 > 50118.72 nM -
CHEMBL3827434 IC50 > 50118.72 nM -
CHEMBL3828357 IC50 > 50118.72 nM -
CHEMBL3828426 IC50 > 50118.72 nM -
CHEMBL3828684 IC50 > 50118.72 nM -
CHEMBL3828026 IC50 > 50118.72 nM -
CHEMBL3828354 IC50 > 50118.72 nM -
CHEMBL3356146 IC50 > 50118.72 nM -
CHEMBL3827560 IC50 > 50118.72 nM -