Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3854930

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human COT (66 to 395 residues) expressed in Sf21 cells using 5-Fluo-Ahx-AGAGSGQLIDSNleANSFVGTR-NH2 as substrate after 60 mins by caliper microfluidic mobility shift assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase kinase kinase 8 (CHEMBL4899)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Imidazoquinolines as a Novel Class of Potent, Selective, and in Vivo Efficacious Cancer Osaka Thyroid (COT) Kinase Inhibitors.
Glatthar R, Stojanovic A, Troxler T, Mattes H, Möbitz H, Beerli R, Blanz J, Gassmann E, Drückes P, Fendrich G, Gutmann S, Martiny-Baron G, Spence F, Hornfeld J, Peel JE, Sparrer H.
J Med Chem (2016) 59 : 7544 -7560
PubMed 27502541 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 7.03 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3912476 1 8.40
CHEMBL3939592 1 8.22
CHEMBL3890505 1 8.05
CHEMBL3927501 1 8.05
CHEMBL3899461 1 7.80
CHEMBL3910848 1 7.70
CHEMBL3918552 1 7.66
CHEMBL3944315 1 7.42
CHEMBL3980906 1 7.40
CHEMBL3937721 1 7.30
CHEMBL3936462 1 7.10
CHEMBL3919207 1 7.05
CHEMBL3908516 1 7.02
CHEMBL3966844 1 7.00
CHEMBL3928098 1 6.82
CHEMBL3955726 1 6.80
CHEMBL3910291 1 6.70
CHEMBL3937086 1 6.17
CHEMBL3909782 1 6.10
CHEMBL3911325 1 5.89
CHEMBL3987154 1 5.89
CHEMBL3928698 1 5.58
CHEMBL3981321 1 5.46
CHEMBL3902393 1 -
CHEMBL507361 MIRDAMETINIB 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3912476 IC50 = 4.0 nM 8.40
CHEMBL3939592 IC50 = 6.0 nM 8.22
CHEMBL3890505 IC50 = 9.0 nM 8.05
CHEMBL3927501 IC50 = 9.0 nM 8.05
CHEMBL3899461 IC50 = 16.0 nM 7.80
CHEMBL3910848 IC50 = 20.0 nM 7.70
CHEMBL3918552 IC50 = 22.0 nM 7.66
CHEMBL3944315 IC50 = 38.0 nM 7.42
CHEMBL3980906 IC50 = 40.0 nM 7.40
CHEMBL3937721 IC50 = 50.0 nM 7.30
CHEMBL3936462 IC50 = 80.0 nM 7.10
CHEMBL3919207 IC50 = 90.0 nM 7.05
CHEMBL3908516 IC50 = 96.0 nM 7.02
CHEMBL3966844 IC50 = 100.0 nM 7.00
CHEMBL3928098 IC50 = 150.0 nM 6.82
CHEMBL3955726 IC50 = 160.0 nM 6.80
CHEMBL3910291 IC50 = 200.0 nM 6.70
CHEMBL3937086 IC50 = 670.0 nM 6.17
CHEMBL3909782 IC50 = 800.0 nM 6.10
CHEMBL3911325 IC50 = 1300.0 nM 5.89
CHEMBL3987154 IC50 = 1300.0 nM 5.89
CHEMBL3928698 IC50 = 2600.0 nM 5.58
CHEMBL3981321 IC50 = 3500.0 nM 5.46
CHEMBL3902393 IC50 > 10000.0 nM -
CHEMBL507361 MIRDAMETINIB IC50 > 25000.0 nM -