Assay Detail
Binding
CHEMBL3856334
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human GST-tagged SIRT1 (18 to 340 residues) expressed in Escherichia coli BL21 (DE3)LysS using acetylated HeLa histones as substrate measured after 2 hrs in presence of NAD by scintillation counting method
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
NAD-dependent protein deacetylase sirtuin-2 (CHEMBL4462) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
How much successful are the medicinal chemists in modulation of SIRT1: A critical review.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 4.42 | 4.42 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL380797 | SIRTINOL | — | 1 | 4.42 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL380797 | SIRTINOL | IC50 | = | 38000.0 | nM | 4.42 |