Assay Detail
Binding
CHEMBL3856824
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant Coxsackievirus B3 3C protease expressed in Escherichia coli BL21 (DE3) preincubated for 5 mins followed by addition of NMA-EALFQGPPVK-DNP-rrr-NH2 as substrate measured every 10 mins for 2 hrs by FRET-based enzyme assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Coxsackievirus B3 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Genome polyprotein (CHEMBL2396505) ↗| Type | SINGLE PROTEIN |
| Organism | Coxsackievirus B3 (strain Nancy) |
Publication
2,3,4-Trihydroxybenzyl-hydrazide analogues as novel potent coxsackievirus B3 3C protease inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 5.60 | 5.62 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1096979 | BENSERAZIDE | 3.0 | 1 | 5.62 |
| CHEMBL3945987 | — | — | 1 | 5.57 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1096979 | BENSERAZIDE | IC50 | = | 2400.0 | nM | 5.62 |
| CHEMBL3945987 | — | IC50 | = | 2700.0 | nM | 5.57 |