Assay Detail
Binding
CHEMBL3856907
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Wistar rat testicular C17,20-lyase assessed as androst-4-ene-3,17-dione formation using [3H]17-hydroxyprogesterone as substrate in presence of NADPH
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Rattus norvegicus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Steroid 17-alpha-hydroxylase/17,20 lyase (CHEMBL4430) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Synthesis of novel 17-(4'-formyl)pyrazolylandrosta-5,16-dienes and their derivatives as potent 17α-hydroxylase/C17,20-lyase inhibitors or antiproliferative agents depending on the substitution pattern of the heteroring.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 7.39 | 8.11 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2112517 | — | — | 1 | 8.11 |
| CHEMBL254328 | ABIRATERONE | 3.0 | 1 | 7.90 |
| CHEMBL3966872 | — | — | 1 | 7.58 |
| CHEMBL3912227 | — | — | 1 | 7.18 |
| CHEMBL3947033 | — | — | 1 | 7.04 |
| CHEMBL157101 | KETOCONAZOLE | 4.0 | 1 | 6.50 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2112517 | — | IC50 | = | 7.7 | nM | 8.11 |
| CHEMBL254328 | ABIRATERONE | IC50 | = | 12.5 | nM | 7.90 |
| CHEMBL3966872 | — | IC50 | = | 26.0 | nM | 7.58 |
| CHEMBL3912227 | — | IC50 | = | 66.0 | nM | 7.18 |
| CHEMBL3947033 | — | IC50 | = | 92.0 | nM | 7.04 |
| CHEMBL157101 | KETOCONAZOLE | IC50 | = | 320.0 | nM | 6.50 |