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Assay Detail

CHEMBL3860146

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured for 5 mins by Ellman's method
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL220)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

New cinnamic - N-benzylpiperidine and cinnamic - N,N-dibenzyl(N-methyl)amine hybrids as Alzheimer-directed multitarget drugs with antioxidant, cholinergic, neuroprotective and neurogenic properties.
Estrada M, Herrera-Arozamena C, Pérez C, Viña D, Romero A, Morales-García JA, Pérez-Castillo A, Rodríguez-Franco MI.
Eur J Med Chem (2016) 121 : 376 -386
PubMed 27267007 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 5.94 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL502 DONEPEZIL 4.0 1 8.00
CHEMBL3818689 1 6.58
CHEMBL3818089 1 6.41
CHEMBL3905695 1 6.31
CHEMBL3916769 1 6.20
CHEMBL3818512 1 6.12
CHEMBL3965783 1 6.00
CHEMBL3819320 1 6.00
CHEMBL3892044 1 5.95
CHEMBL3818992 1 5.76
CHEMBL3949439 1 5.46
CHEMBL3917990 1 5.40
CHEMBL3919086 1 5.33
CHEMBL3978533 1 5.24
CHEMBL3945872 1 5.22
CHEMBL3936989 1 5.06
CHEMBL972 SELEGILINE 4.0 1 -
CHEMBL153 TROLOX 1 -
CHEMBL86304 MOCLOBEMIDE 4.0 1 -
CHEMBL92401 IPRONIAZID 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL502 DONEPEZIL IC50 = 10.0 nM 8.00
CHEMBL3818689 IC50 = 260.0 nM 6.58
CHEMBL3818089 IC50 = 390.0 nM 6.41
CHEMBL3905695 IC50 = 490.0 nM 6.31
CHEMBL3916769 IC50 = 630.0 nM 6.20
CHEMBL3818512 IC50 = 750.0 nM 6.12
CHEMBL3965783 IC50 = 990.0 nM 6.00
CHEMBL3819320 IC50 = 1010.0 nM 6.00
CHEMBL3892044 IC50 = 1120.0 nM 5.95
CHEMBL3818992 IC50 = 1750.0 nM 5.76
CHEMBL3949439 IC50 = 3490.0 nM 5.46
CHEMBL3917990 IC50 = 3980.0 nM 5.40
CHEMBL3919086 IC50 = 4670.0 nM 5.33
CHEMBL3978533 IC50 = 5760.0 nM 5.24
CHEMBL3945872 IC50 = 5990.0 nM 5.22
CHEMBL3936989 IC50 = 8730.0 nM 5.06
CHEMBL972 SELEGILINE IC50 - -
CHEMBL153 TROLOX IC50 - -
CHEMBL86304 MOCLOBEMIDE IC50 - -
CHEMBL92401 IPRONIAZID IC50 - -