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Assay Detail

CHEMBL3860367

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal 6x-HIS-tagged human recombinant EGFR (696 to 1022 residues) L858R/T790M mutant expressed in Sf9 cells pre-incubated for 90 mins followed by 1 mM ATP addition by HTRF assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of (R,E)-N-(7-Chloro-1-(1-[4-(dimethylamino)but-2-enoyl]azepan-3-yl)-1H-benzo[d]imidazol-2-yl)-2-methylisonicotinamide (EGF816), a Novel, Potent, and WT Sparing Covalent Inhibitor of Oncogenic (L858R, ex19del) and Resistant (T790M) EGFR Mutants for the Treatment of EGFR Mutant Non-Small-Cell Lung Cancers.
Lelais G, Epple R, Marsilje TH, Long YO, McNeill M, Chen B, Lu W, Anumolu J, Badiger S, Bursulaya B, DiDonato M, Fong R, Juarez J, Li J, Manuia M, Mason DE, Gordon P, Groessl T, Johnson K, Jia Y, Kasibhatla S, Li C, Isbell J, Spraggon G, Bender S, Michellys PY.
J Med Chem (2016) 59 : 6671 -6689
PubMed 27433829 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 7.12 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3953221 1 8.40
CHEMBL3959632 1 8.40
CHEMBL3961202 1 8.00
CHEMBL3953921 1 7.70
CHEMBL3966111 1 7.30
CHEMBL3973973 1 7.22
CHEMBL3892960 1 5.17
CHEMBL3982602 1 4.78
CHEMBL3921545 1 -
CHEMBL3894391 1 -
CHEMBL3901943 1 -
CHEMBL3972987 1 -
CHEMBL3961970 1 -
CHEMBL3981978 1 -
CHEMBL3944524 1 -
CHEMBL3962999 1 -
CHEMBL3935558 1 -
CHEMBL3936272 1 -
CHEMBL3916436 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3953221 IC50 = 4.0 nM 8.40
CHEMBL3959632 IC50 = 4.0 nM 8.40
CHEMBL3961202 IC50 = 10.0 nM 8.00
CHEMBL3953921 IC50 = 20.0 nM 7.70
CHEMBL3966111 IC50 = 50.0 nM 7.30
CHEMBL3973973 IC50 = 60.0 nM 7.22
CHEMBL3892960 IC50 = 6810.0 nM 5.17
CHEMBL3982602 IC50 = 16460.0 nM 4.78
CHEMBL3921545 IC50 > 50000.0 nM -
CHEMBL3894391 IC50 > 50000.0 nM -
CHEMBL3901943 IC50 < 2.0 nM -
CHEMBL3972987 IC50 > 50000.0 nM -
CHEMBL3961970 IC50 > 50000.0 nM -
CHEMBL3981978 IC50 > 50000.0 nM -
CHEMBL3944524 IC50 > 50000.0 nM -
CHEMBL3962999 IC50 > 50000.0 nM -
CHEMBL3935558 IC50 > 32770.0 nM -
CHEMBL3936272 IC50 > 50000.0 nM -
CHEMBL3916436 IC50 > 50000.0 nM -