Assay Detail
Binding
CHEMBL3862298
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Inhibition of human recombinant BuChE expressed in HEK293 cells using S-butyrylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition measured after 30 mins by Ellman's method
11
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Donepezil-like multifunctional agents: Design, synthesis, molecular modeling and biological evaluation.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 7.05 | 8.70 |
| Ki | 1 | 7.28 | 7.28 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3972214 | — | — | 1 | 8.70 |
| CHEMBL3961103 | — | — | 1 | 7.64 |
| CHEMBL3901082 | — | — | 1 | 7.52 |
| CHEMBL3910142 | — | — | 2 | 7.41 |
| CHEMBL3931599 | — | — | 1 | 7.19 |
| CHEMBL3892118 | — | — | 1 | 7.10 |
| CHEMBL3291019 | — | — | 1 | 5.68 |
| CHEMBL502 | DONEPEZIL | 4.0 | 1 | 5.12 |
| CHEMBL8706 | CLORGILINE | 2.0 | 1 | - |
| CHEMBL972 | SELEGILINE | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3972214 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3961103 | — | IC50 | = | 23.0 | nM | 7.64 |
| CHEMBL3901082 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL3910142 | — | IC50 | = | 39.0 | nM | 7.41 |
| CHEMBL3910142 | — | Ki | = | 52.0 | nM | 7.28 |
| CHEMBL3931599 | — | IC50 | = | 64.0 | nM | 7.19 |
| CHEMBL3892118 | — | IC50 | = | 79.0 | nM | 7.10 |
| CHEMBL3291019 | — | IC50 | = | 2100.0 | nM | 5.68 |
| CHEMBL502 | DONEPEZIL | IC50 | = | 7500.0 | nM | 5.12 |
| CHEMBL8706 | CLORGILINE | IC50 | > | 500000.0 | nM | - |
| CHEMBL972 | SELEGILINE | IC50 | > | 500000.0 | nM | - |