Assay Detail
Binding
CHEMBL3862876
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Inhibition of hedgehog signaling pathway in mouse Shh Light2 cells assessed as inhibition of sonic hedgehog-induced GLI1-mediated transcriptional activity measured after 30 hrs by dual luciferase reporter gene assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | Shh Light2 |
| Confidence | 3 — Cell-line / Tissue |
| Curated By | Autocuration |
Publication
Three new alkaloids from Veratrum grandiflorum Loes with inhibition activities on Hedgehog pathway.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 6.07 | 7.13 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL254129 | CYCLOPAMINE | — | 1 | 7.13 |
| CHEMBL3933007 | — | — | 1 | 6.20 |
| CHEMBL3906057 | — | — | 1 | 5.96 |
| CHEMBL3974090 | — | — | 1 | 5.56 |
| CHEMBL3945487 | — | — | 1 | 5.51 |
| CHEMBL3943503 | — | — | 1 | - |
| CHEMBL1980466 | — | — | 1 | - |
| CHEMBL3914058 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL254129 | CYCLOPAMINE | IC50 | = | 74.1 | nM | 7.13 |
| CHEMBL3933007 | — | IC50 | = | 630.0 | nM | 6.20 |
| CHEMBL3906057 | — | IC50 | = | 1110.0 | nM | 5.96 |
| CHEMBL3974090 | — | IC50 | = | 2780.0 | nM | 5.56 |
| CHEMBL3945487 | — | IC50 | = | 3110.0 | nM | 5.51 |
| CHEMBL3943503 | — | IC50 | > | 25000.0 | nM | - |
| CHEMBL1980466 | — | IC50 | > | 25000.0 | nM | - |
| CHEMBL3914058 | — | IC50 | > | 25000.0 | nM | - |