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Assay Detail

CHEMBL3863085

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Intrinsic activity at human alpha1B adrenergic receptor expressed in CHOK1 cells co-expressing aequorin assessed as inhibition of agonist induced calcium mobilization preincubated for 15 mins followed by agonist addition measured for 20 secs in presence of coelenterazine H by luminescence assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Alpha-1B adrenergic receptor (CHEMBL232)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Arylsulfonamide derivatives of (aryloxy)ethyl pyrrolidines and piperidines as α1-adrenergic receptor antagonist with uro-selective activity.
Rak A, Canale V, Marciniec K, Żmudzki P, Kotańska M, Knutelska J, Siwek A, Stachowicz G, Bednarski M, Nowiński L, Zygmunt M, Zajdel P, Sapa J.
Bioorg Med Chem (2016) 24 : 5582 -5591
PubMed 27658792 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 11 7.42 9.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL836 TAMSULOSIN 4.0 1 9.89
CHEMBL611 TERAZOSIN 4.0 1 8.76
CHEMBL3937911 1 7.90
CHEMBL3895815 1 7.85
CHEMBL3956998 1 7.81
CHEMBL3947830 1 7.38
CHEMBL3894175 1 6.98
CHEMBL3948333 1 6.34
CHEMBL3986337 1 6.29
CHEMBL3939560 1 6.22
CHEMBL3968662 1 6.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL836 TAMSULOSIN EC50 = 0.13 nM 9.89
CHEMBL611 TERAZOSIN EC50 = 1.73 nM 8.76
CHEMBL3937911 EC50 = 12.65 nM 7.90
CHEMBL3895815 EC50 = 14.15 nM 7.85
CHEMBL3956998 EC50 = 15.5 nM 7.81
CHEMBL3947830 EC50 = 42.1 nM 7.38
CHEMBL3894175 EC50 = 104.8 nM 6.98
CHEMBL3948333 EC50 = 461.1 nM 6.34
CHEMBL3986337 EC50 = 514.8 nM 6.29
CHEMBL3939560 EC50 = 608.4 nM 6.22
CHEMBL3968662 EC50 = 598.6 nM 6.22