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Assay Detail

CHEMBL3863934

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Shigella dysenteriae type 1 Shiga toxin A subunit assessed as change in transition temperature by SYPRO orange dye based fluorescence-based thermal shift assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Shigella dysenteriae
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Shiga toxin subunit A (CHEMBL3879822)
Type SINGLE PROTEIN
Organism Shigella dysenteriae

Publication

In Silico Discovery and Validation of Amide Based Small Molecule Targeting the Enzymatic Site of Shiga Toxin.
Chauhan V, Chaudhary D, Pathak U, Saxena N, Dhaked RK.
J Med Chem (2016) 59 : 10763 -10773
PubMed 27933947 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 20 4.82 5.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3933587 1 5.49
CHEMBL3939786 1 5.47
CHEMBL2375419 1 5.39
CHEMBL3917910 1 5.30
CHEMBL1253710 1 5.22
CHEMBL3935227 1 5.17
CHEMBL3964948 1 5.06
CHEMBL241862 1 5.00
CHEMBL227423 1 4.96
CHEMBL3952356 1 4.88
CHEMBL2336353 N-(2-HYDROXYPHENYL)CINNAMAMIDE 1 4.73
CHEMBL3889898 1 4.46
CHEMBL3934770 1 4.43
CHEMBL590801 1 4.38
CHEMBL3963596 1 4.37
CHEMBL3955063 1 4.27
CHEMBL3895016 1 4.13
CHEMBL3942549 1 4.03
CHEMBL3924676 1 -
CHEMBL250859 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3933587 Ki = 3210.0 nM 5.49
CHEMBL3939786 Ki = 3360.0 nM 5.47
CHEMBL2375419 Ki = 4100.0 nM 5.39
CHEMBL3917910 Ki = 5000.0 nM 5.30
CHEMBL1253710 Ki = 5960.0 nM 5.22
CHEMBL3935227 Ki = 6690.0 nM 5.17
CHEMBL3964948 Ki = 8780.0 nM 5.06
CHEMBL241862 Ki = 9930.0 nM 5.00
CHEMBL227423 Ki = 10880.0 nM 4.96
CHEMBL3952356 Ki = 13330.0 nM 4.88
CHEMBL2336353 N-(2-HYDROXYPHENYL)CINNAMAMIDE Ki = 18680.0 nM 4.73
CHEMBL3889898 Ki = 34500.0 nM 4.46
CHEMBL3934770 Ki = 37050.0 nM 4.43
CHEMBL590801 Ki = 41990.0 nM 4.38
CHEMBL3963596 Ki = 42460.0 nM 4.37
CHEMBL3955063 Ki = 53780.0 nM 4.27
CHEMBL3895016 Ki = 73780.0 nM 4.13
CHEMBL3942549 Ki = 93860.0 nM 4.03
CHEMBL3924676 Ki = 189360.0 nM -
CHEMBL250859 Ki = 439630.0 nM -