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Assay Detail

CHEMBL3864050

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NLRP3 inflammasome activation in LPS-stimulated mouse BMDM assessed as reduction in NLRP3 inflammasome-induced IL-1beta production preincubated for 30 mins followed by NLRP3 stimulation with ATP by ELISA
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

NACHT, LRR and PYD domains-containing protein 3 (CHEMBL3779755)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Identification, Synthesis, and Biological Evaluation of the Major Human Metabolite of NLRP3 Inflammasome Inhibitor MCC950.
Salla M, Butler MS, Pelingon R, Kaeslin G, Croker DE, Reid JC, Baek JM, Bernhardt PV, Gillam EM, Cooper MA, Robertson AA.
ACS Med Chem Lett (2016) 7 : 1034 -1038
PubMed 27994733 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 8.12 8.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3183703 1 8.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3183703 IC50 = 7.5 nM 8.12