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Assay Detail

CHEMBL3865005

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Hepatitis C virus genotype 2b replicon NS5A transfected in human HuH7 cells assessed as decrease in replication of viral replicon after 4 days by luciferase reporter gene assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus subtype 2b
Cell Type Huh-7
Confidence 1 — Organism
Curated By Autocuration

Publication

Discovery of fluorobenzimidazole HCV NS5A inhibitors.
Randolph JT, Flentge CA, Donner P, Rockway TW, Patel SV, Nelson L, Hutchinson DK, Mondal R, Mistry N, Reisch T, Dekhtyar T, Krishnan P, Pilot-Matias T, Stolarik DF, Beno DWA, Wagner R, Maring C, Kati WM.
Bioorg Med Chem Lett (2016) 26 : 5462 -5467
PubMed 27780635 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 23 9.09 11.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3960757 1 11.00
CHEMBL3892720 1 10.60
CHEMBL3891600 1 10.42
CHEMBL3121846 1 10.22
CHEMBL3965250 1 9.83
CHEMBL3899984 1 9.66
CHEMBL3944264 1 9.65
CHEMBL3979298 1 9.49
CHEMBL3981757 1 9.29
CHEMBL3960050 1 8.85
CHEMBL3980231 1 8.41
CHEMBL3961720 1 8.35
CHEMBL3898584 1 8.19
CHEMBL3889915 1 7.25
CHEMBL3917933 1 7.11
CHEMBL3953012 1 7.05
CHEMBL3973743 1 -
CHEMBL3899945 1 -
CHEMBL3962172 1 -
CHEMBL3919651 1 -
CHEMBL3985488 1 -
CHEMBL3915742 1 -
CHEMBL3977398 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3960757 EC50 = 0.01 nM 11.00
CHEMBL3892720 EC50 = 0.025 nM 10.60
CHEMBL3891600 EC50 = 0.038 nM 10.42
CHEMBL3121846 EC50 = 0.06 nM 10.22
CHEMBL3965250 EC50 = 0.148 nM 9.83
CHEMBL3899984 EC50 = 0.218 nM 9.66
CHEMBL3944264 EC50 = 0.222 nM 9.65
CHEMBL3979298 EC50 = 0.32 nM 9.49
CHEMBL3981757 EC50 = 0.516 nM 9.29
CHEMBL3960050 EC50 = 1.43 nM 8.85
CHEMBL3980231 EC50 = 3.93 nM 8.41
CHEMBL3961720 EC50 = 4.49 nM 8.35
CHEMBL3898584 EC50 = 6.5 nM 8.19
CHEMBL3889915 EC50 = 56.8 nM 7.25
CHEMBL3917933 EC50 = 77.5 nM 7.11
CHEMBL3953012 EC50 = 88.6 nM 7.05
CHEMBL3973743 EC50 < 0.03 nM -
CHEMBL3899945 EC50 > 100.0 nM -
CHEMBL3962172 EC50 > 10.0 nM -
CHEMBL3919651 EC50 = 0.009 nM -
CHEMBL3985488 EC50 = 0.009 nM -
CHEMBL3915742 EC50 = 0.007 nM -
CHEMBL3977398 EC50 = 0.004 nM -