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Assay Detail

CHEMBL3865024

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Hepatitis C virus genotype 6a replicon NS5A transfected in human HuH7 cells assessed as decrease in replication of viral replicon after 4 days by luciferase reporter gene assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus subtype 6a
Cell Type Huh-7
Confidence 1 — Organism
Curated By Autocuration

Publication

Discovery of fluorobenzimidazole HCV NS5A inhibitors.
Randolph JT, Flentge CA, Donner P, Rockway TW, Patel SV, Nelson L, Hutchinson DK, Mondal R, Mistry N, Reisch T, Dekhtyar T, Krishnan P, Pilot-Matias T, Stolarik DF, Beno DWA, Wagner R, Maring C, Kati WM.
Bioorg Med Chem Lett (2016) 26 : 5462 -5467
PubMed 27780635 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 22 8.86 10.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3985488 1 10.82
CHEMBL3919651 1 9.94
CHEMBL3892720 1 9.88
CHEMBL3973743 1 9.56
CHEMBL3891600 1 9.23
CHEMBL3965250 1 8.49
CHEMBL3944264 1 8.32
CHEMBL3979298 1 8.15
CHEMBL3899984 1 7.76
CHEMBL3961720 1 7.67
CHEMBL3980231 1 7.64
CHEMBL3898584 1 -
CHEMBL3960050 1 -
CHEMBL3899945 1 -
CHEMBL3917933 1 -
CHEMBL3889915 1 -
CHEMBL3953012 1 -
CHEMBL3981757 1 -
CHEMBL3962172 1 -
CHEMBL3915742 1 -
CHEMBL3121846 1 -
CHEMBL3977398 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3985488 EC50 = 0.015 nM 10.82
CHEMBL3919651 EC50 = 0.114 nM 9.94
CHEMBL3892720 EC50 = 0.132 nM 9.88
CHEMBL3973743 EC50 = 0.274 nM 9.56
CHEMBL3891600 EC50 = 0.59 nM 9.23
CHEMBL3965250 EC50 = 3.24 nM 8.49
CHEMBL3944264 EC50 = 4.75 nM 8.32
CHEMBL3979298 EC50 = 7.05 nM 8.15
CHEMBL3899984 EC50 = 17.3 nM 7.76
CHEMBL3961720 EC50 = 21.5 nM 7.67
CHEMBL3980231 EC50 = 22.9 nM 7.64
CHEMBL3898584 EC50 - -
CHEMBL3960050 EC50 - -
CHEMBL3899945 EC50 - -
CHEMBL3917933 EC50 > 100.0 nM -
CHEMBL3889915 EC50 > 100.0 nM -
CHEMBL3953012 EC50 > 100.0 nM -
CHEMBL3981757 EC50 - -
CHEMBL3962172 EC50 > 10.0 nM -
CHEMBL3915742 EC50 = 0.007 nM -
CHEMBL3121846 EC50 - -
CHEMBL3977398 EC50 = 0.007 nM -