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Assay Detail

CHEMBL3869550

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at recombinant human mGlu3 receptor expressed in hamster AV12 cells co-expressing human EAAT1 assessed as reduction of DCG-4-inhibited forskolin-stimulated cAMP formation after 20 mins by HTRF assay
35
Total Activities
18
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type AV12
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Metabotropic glutamate receptor 3 (CHEMBL2888)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel bicyclo[3.1.0]hexane analogs as antagonists of metabotropic glutamate 2/3 receptors for the treatment of depression.
Dressman BA, Tromiczak EG, Chappell MD, Tripp AE, Quimby SJ, Vetman T, Fivush AM, Matt J, Jaramillo C, Li R, Khilevich A, Blanco MJ, Smith SC, Carpintero M, de Diego JE, Barberis M, García-Cerrada S, Soriano JF, Schkeryantz JM, Witkin JM, Wafford KA, Seidel W, Britton T, Overshiner CD, Li X, Wang XS, Heinz BA, Catlow JT, Swanson S, Bedwell D, Ornstein PL, Mitch CH.
Bioorg Med Chem Lett (2016) 26 : 5663 -5668
PubMed 27836401 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.10 7.84
Imax 17 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3913414 2 7.84
CHEMBL3939492 2 7.69
CHEMBL3969063 2 7.63
CHEMBL3948272 2 7.53
CHEMBL3977508 2 7.42
CHEMBL3947221 2 7.34
CHEMBL3933829 2 7.27
CHEMBL3961306 2 7.15
CHEMBL3956934 1 7.12
CHEMBL3918170 2 7.08
CHEMBL3927108 2 7.07
CHEMBL3890112 2 6.90
CHEMBL3971347 2 6.84
CHEMBL3909237 2 6.82
CHEMBL3953570 2 6.75
CHEMBL3979475 2 6.73
CHEMBL3955458 2 6.62
CHEMBL3930511 2 5.97

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3913414 IC50 = 14.3 nM 7.84
CHEMBL3939492 IC50 = 20.5 nM 7.69
CHEMBL3969063 IC50 = 23.5 nM 7.63
CHEMBL3948272 IC50 = 29.6 nM 7.53
CHEMBL3977508 IC50 = 38.5 nM 7.42
CHEMBL3947221 IC50 = 46.1 nM 7.34
CHEMBL3933829 IC50 = 54.1 nM 7.27
CHEMBL3961306 IC50 = 71.0 nM 7.15
CHEMBL3956934 IC50 = 76.1 nM 7.12
CHEMBL3918170 IC50 = 83.2 nM 7.08
CHEMBL3927108 IC50 = 85.0 nM 7.07
CHEMBL3890112 IC50 = 127.0 nM 6.90
CHEMBL3971347 IC50 = 145.0 nM 6.84
CHEMBL3909237 IC50 = 153.0 nM 6.82
CHEMBL3953570 IC50 = 178.0 nM 6.75
CHEMBL3979475 IC50 = 185.0 nM 6.73
CHEMBL3955458 IC50 = 239.0 nM 6.62
CHEMBL3930511 IC50 = 1070.0 nM 5.97
CHEMBL3977508 Imax > 94.0 % -
CHEMBL3955458 Imax > 94.0 % -
CHEMBL3953570 Imax > 94.0 % -
CHEMBL3971347 Imax > 94.0 % -
CHEMBL3909237 Imax > 94.0 % -
CHEMBL3979475 Imax > 94.0 % -
CHEMBL3918170 Imax > 94.0 % -
CHEMBL3890112 Imax > 94.0 % -
CHEMBL3927108 Imax > 94.0 % -
CHEMBL3947221 Imax > 94.0 % -
CHEMBL3961306 Imax > 94.0 % -
CHEMBL3933829 Imax > 94.0 % -
CHEMBL3930511 Imax > 94.0 % -
CHEMBL3913414 Imax > 94.0 % -
CHEMBL3939492 Imax > 94.0 % -
CHEMBL3969063 Imax > 94.0 % -
CHEMBL3948272 Imax > 94.0 % -