Assay Detail
Binding
CHEMBL3870650
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of wild type recombinant Atg4B (unknown origin) expressed in Escherichia coli BL21 DE3 using N-terminal His6-tagged LC3B-PLA2 as substrate after 60 mins by NBD-C6-HPC dye-based fluorescence assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Inhibitor screening and enzymatic activity determination for autophagy target Atg4B using a gel electrophoresis-based assay.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 5.80 | 6.50 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1496397 | — | — | 1 | 6.50 |
| CHEMBL559337 | 7-DESHYDROXYPYROGALLIN-4-CARBOXYLIC ACID | — | 1 | 5.64 |
| CHEMBL6246 | ELLAGIC ACID | 2.0 | 1 | 5.26 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1496397 | — | IC50 | = | 316.0 | nM | 6.50 |
| CHEMBL559337 | 7-DESHYDROXYPYROGALLIN-4-CARBOXYLIC ACID | IC50 | = | 2300.0 | nM | 5.64 |
| CHEMBL6246 | ELLAGIC ACID | IC50 | = | 5500.0 | nM | 5.26 |