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Assay Detail

CHEMBL3871160

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Positive allosteric modulation of GluN1/GluN2A receptor (unknown origin) expressed in CHO cells assessed as increase in glutamate-induced calcium flux measured at time interval of 5 mins in presence of saturating glycine by calcium dye-based fluorescence assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Glutamate NMDA receptor; GRIN1/GRIN2A (CHEMBL1907604)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

GluN2A-Selective Pyridopyrimidinone Series of NMDAR Positive Allosteric Modulators with an Improved in Vivo Profile.
Villemure E, Volgraf M, Jiang Y, Wu G, Ly CQ, Yuen PW, Lu A, Luo X, Liu M, Zhang S, Lupardus PJ, Wallweber HJ, Liederer BM, Deshmukh G, Plise E, Tay S, Wang TM, Hanson JE, Hackos DH, Scearce-Levie K, Schwarz JB, Sellers BD.
ACS Med Chem Lett (2017) 8 : 84 -89
PubMed 28105280 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 15 6.79 7.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3970291 1 7.68
CHEMBL3891918 1 7.62
CHEMBL3799878 1 7.52
CHEMBL3938522 1 7.43
CHEMBL3976578 1 7.41
CHEMBL3983039 1 7.39
CHEMBL3968067 1 7.10
CHEMBL3948381 1 6.92
CHEMBL3944810 1 6.89
CHEMBL3945685 1 6.57
CHEMBL3975050 1 6.55
CHEMBL3799694 1 6.36
CHEMBL3985768 1 5.85
CHEMBL3955420 1 5.60
CHEMBL3966561 1 5.01

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3970291 EC50 = 21.0 nM 7.68
CHEMBL3891918 EC50 = 24.0 nM 7.62
CHEMBL3799878 EC50 = 30.0 nM 7.52
CHEMBL3938522 EC50 = 37.0 nM 7.43
CHEMBL3976578 EC50 = 39.0 nM 7.41
CHEMBL3983039 EC50 = 41.0 nM 7.39
CHEMBL3968067 EC50 = 80.0 nM 7.10
CHEMBL3948381 EC50 = 120.0 nM 6.92
CHEMBL3944810 EC50 = 130.0 nM 6.89
CHEMBL3945685 EC50 = 270.0 nM 6.57
CHEMBL3975050 EC50 = 280.0 nM 6.55
CHEMBL3799694 EC50 = 440.0 nM 6.36
CHEMBL3985768 EC50 = 1400.0 nM 5.85
CHEMBL3955420 EC50 = 2500.0 nM 5.60
CHEMBL3966561 EC50 = 9800.0 nM 5.01