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Assay Detail

CHEMBL3873676

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length recombinant human N-terminal His6-tagged PYK2 expressed in baculovirus infected sf21 cells
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein-tyrosine kinase 2-beta (CHEMBL5469)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selectivity switch between FAK and Pyk2: Macrocyclization of FAK inhibitors improves Pyk2 potency.
Farand J, Mai N, Chandrasekhar J, Newby ZE, Van Veldhuizen J, Loyer-Drew J, Venkataramani C, Guerrero J, Kwok A, Li N, Zherebina Y, Wilbert S, Zablocki J, Phillips G, Watkins WJ, Mourey R, Notte GT.
Bioorg Med Chem Lett (2016) 26 : 5926 -5930
PubMed 27876318 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 8.04 9.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3949755 1 9.17
CHEMBL3958414 1 9.08
CHEMBL3956954 1 9.05
CHEMBL3987106 1 8.88
CHEMBL3899046 1 8.82
CHEMBL3969464 1 8.81
CHEMBL3908093 1 8.72
CHEMBL3961368 1 8.59
CHEMBL3977870 1 8.57
CHEMBL3889951 1 8.51
CHEMBL3912281 1 8.46
CHEMBL1084546 PF-00562271 1.0 1 7.89
CHEMBL3965256 1 7.71
CHEMBL3939286 1 7.58
CHEMBL3914036 1 7.52
CHEMBL3892377 1 6.91
CHEMBL3927099 1 6.70
CHEMBL3936048 1 6.59
CHEMBL3898282 1 5.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3949755 IC50 = 0.67 nM 9.17
CHEMBL3958414 IC50 = 0.84 nM 9.08
CHEMBL3956954 IC50 = 0.9 nM 9.05
CHEMBL3987106 IC50 = 1.31 nM 8.88
CHEMBL3899046 IC50 = 1.5 nM 8.82
CHEMBL3969464 IC50 = 1.55 nM 8.81
CHEMBL3908093 IC50 = 1.9 nM 8.72
CHEMBL3961368 IC50 = 2.6 nM 8.59
CHEMBL3977870 IC50 = 2.7 nM 8.57
CHEMBL3889951 IC50 = 3.1 nM 8.51
CHEMBL3912281 IC50 = 3.5 nM 8.46
CHEMBL1084546 PF-00562271 IC50 = 13.0 nM 7.89
CHEMBL3965256 IC50 = 19.5 nM 7.71
CHEMBL3939286 IC50 = 26.0 nM 7.58
CHEMBL3914036 IC50 = 30.0 nM 7.52
CHEMBL3892377 IC50 = 122.0 nM 6.91
CHEMBL3927099 IC50 = 201.0 nM 6.70
CHEMBL3936048 IC50 = 256.0 nM 6.59
CHEMBL3898282 IC50 = 6625.0 nM 5.18