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Assay Detail

CHEMBL3875847

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Binding
Inhibition of recombinant human GFP-fused ABCG2 expressed in MDCK2 cells assessed as reduction in pheophorbide A efflux preincubated for 30 mins followed by pheophorbide A addition measured after 2 hrs by flow cytometry
32
Total Activities
32
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MDCK-II
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Phenyltetrazolyl-phenylamides: Substituent impact on modulation capability and selectivity toward the efflux protein ABCG2 and investigation of interaction with the transporter.
Köhler SC, Silbermann K, Wiese M.
Eur J Med Chem (2016) 124 : 881 -895
PubMed 27676469 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 32 6.57 7.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3884089 1 7.19
CHEMBL3883513 1 7.01
CHEMBL3885113 1 6.93
CHEMBL3884853 1 6.91
CHEMBL3883590 1 6.89
CHEMBL3885031 1 6.88
CHEMBL3883987 1 6.68
CHEMBL3885018 1 6.67
CHEMBL3884824 1 6.61
CHEMBL3885364 1 6.60
CHEMBL488910 1 6.56
CHEMBL3885456 1 6.52
CHEMBL3883387 1 6.51
CHEMBL3885022 1 6.49
CHEMBL3883389 1 6.34
CHEMBL3885212 1 6.29
CHEMBL3883929 1 6.17
CHEMBL428402 1 6.13
CHEMBL3884050 1 5.44
CHEMBL3884305 1 -
CHEMBL3884145 1 -
CHEMBL3885424 1 -
CHEMBL3884164 1 -
CHEMBL3883933 1 -
CHEMBL3885414 1 -
CHEMBL3883221 1 -
CHEMBL3885158 1 -
CHEMBL3884827 1 -
CHEMBL3883362 1 -
CHEMBL3884587 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3884089 IC50 = 64.0 nM 7.19
CHEMBL3883513 IC50 = 96.6 nM 7.01
CHEMBL3885113 IC50 = 117.0 nM 6.93
CHEMBL3884853 IC50 = 123.0 nM 6.91
CHEMBL3883590 IC50 = 129.0 nM 6.89
CHEMBL3885031 IC50 = 131.0 nM 6.88
CHEMBL3883987 IC50 = 208.0 nM 6.68
CHEMBL3885018 IC50 = 212.0 nM 6.67
CHEMBL3884824 IC50 = 247.0 nM 6.61
CHEMBL3885364 IC50 = 251.0 nM 6.60
CHEMBL488910 IC50 = 276.0 nM 6.56
CHEMBL3885456 IC50 = 300.0 nM 6.52
CHEMBL3883387 IC50 = 311.0 nM 6.51
CHEMBL3885022 IC50 = 321.0 nM 6.49
CHEMBL3883389 IC50 = 458.0 nM 6.34
CHEMBL3885212 IC50 = 511.0 nM 6.29
CHEMBL3883929 IC50 = 679.0 nM 6.17
CHEMBL428402 IC50 = 741.0 nM 6.13
CHEMBL3884050 IC50 = 3630.0 nM 5.44
CHEMBL3884305 IC50 - -
CHEMBL3884145 IC50 - -
CHEMBL3885424 IC50 - -
CHEMBL3884164 IC50 - -
CHEMBL3883933 IC50 - -
CHEMBL3885414 IC50 - -
CHEMBL3883221 IC50 - -
CHEMBL3885158 IC50 - -
CHEMBL3884827 IC50 - -
CHEMBL3883362 IC50 - -
CHEMBL3884587 IC50 - -
CHEMBL3885198 IC50 - -
CHEMBL3885165 IC50 - -