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Assay Detail

CHEMBL3876529

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Positive allosteric modulation of human mGlu2 receptor expressed in HEK293 cells coexpressing rat glutamate transporter assessed as inhibition of forskolin-stimulated cAMP production measured after 30 mins in presence of orthosteric antagonist LY341495 by FLIPR assay
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Metabotropic glutamate receptor 2 (CHEMBL5137)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Triazolopyridine ethers as potent, orally active mGlu2 positive allosteric modulators for treating schizophrenia.
Higgins MA, Marcin LR, Christopher Zusi F, Gentles R, Ding M, Pearce BC, Easton A, Kostich WA, Seager MA, Bourin C, Bristow LJ, Johnson KA, Miller R, Hogan J, Whiterock V, Gulianello M, Ferrante M, Huang Y, Hendricson A, Alt A, Macor JE, Bronson JJ.
Bioorg Med Chem (2017) 25 : 496 -513
PubMed 27914948 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 29 8.02 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3883435 1 9.40
CHEMBL3884532 1 9.40
CHEMBL3884187 1 9.30
CHEMBL3884636 1 8.89
CHEMBL3885379 1 8.85
CHEMBL3883634 1 8.77
CHEMBL3884094 1 8.74
CHEMBL3884833 1 8.40
CHEMBL3884936 1 8.26
CHEMBL3885115 1 8.21
CHEMBL3883801 1 8.17
CHEMBL3884343 1 8.00
CHEMBL3884075 1 7.96
CHEMBL3884332 1 7.96
CHEMBL3885396 1 7.89
CHEMBL3885312 1 7.89
CHEMBL3885265 1 7.80
CHEMBL3885097 1 7.77
CHEMBL3885072 1 7.75
CHEMBL3885277 1 7.70
CHEMBL3885353 1 7.55
CHEMBL3883585 1 7.54
CHEMBL3883827 1 7.50
CHEMBL3884170 1 7.22
CHEMBL3885492 1 6.75
CHEMBL3885493 1 6.64
CHEMBL3884042 1 6.14
CHEMBL3883462 1 -
CHEMBL3883487 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3883435 EC50 = 0.4 nM 9.40
CHEMBL3884532 EC50 = 0.4 nM 9.40
CHEMBL3884187 EC50 = 0.5 nM 9.30
CHEMBL3884636 EC50 = 1.3 nM 8.89
CHEMBL3885379 EC50 = 1.4 nM 8.85
CHEMBL3883634 EC50 = 1.7 nM 8.77
CHEMBL3884094 EC50 = 1.8 nM 8.74
CHEMBL3884833 EC50 = 4.0 nM 8.40
CHEMBL3884936 EC50 = 5.5 nM 8.26
CHEMBL3885115 EC50 = 6.1 nM 8.21
CHEMBL3883801 EC50 = 6.8 nM 8.17
CHEMBL3884343 EC50 = 10.0 nM 8.00
CHEMBL3884075 EC50 = 11.0 nM 7.96
CHEMBL3884332 EC50 = 11.0 nM 7.96
CHEMBL3885396 EC50 = 13.0 nM 7.89
CHEMBL3885312 EC50 = 13.0 nM 7.89
CHEMBL3885265 EC50 = 16.0 nM 7.80
CHEMBL3885097 EC50 = 17.0 nM 7.77
CHEMBL3885072 EC50 = 18.0 nM 7.75
CHEMBL3885277 EC50 = 20.0 nM 7.70
CHEMBL3885353 EC50 = 28.0 nM 7.55
CHEMBL3883585 EC50 = 29.0 nM 7.54
CHEMBL3883827 EC50 = 32.0 nM 7.50
CHEMBL3884170 EC50 = 60.0 nM 7.22
CHEMBL3885492 EC50 = 180.0 nM 6.75
CHEMBL3885493 EC50 = 230.0 nM 6.64
CHEMBL3884042 EC50 = 720.0 nM 6.14
CHEMBL3883462 EC50 > 3000.0 nM -
CHEMBL3883487 EC50 > 15000.0 nM -