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Assay Detail

CHEMBL3877019

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-8-OH-DPAT from recombinant human 5-HT1A receptor expressed in CHOK1 cell membranes after 1 hr by liquid scintillation counting method
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 1A (CHEMBL214)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

New N- and O-arylpiperazinylalkyl pyrimidines and 2-methylquinazolines derivatives as 5-HT7 and 5-HT1A receptor ligands: Synthesis, structure-activity relationships, and molecular modeling studies.
Intagliata S, Modica MN, Pittalà V, Salerno L, Siracusa MA, Cagnotto A, Salmona M, Kurczab R, Romeo G.
Bioorg Med Chem (2017) 25 : 1250 -1259
PubMed 28063784 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 7.30 8.58

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL56 8-OH-DPAT 1 8.58
CHEMBL3884095 1 8.52
CHEMBL3883640 1 8.16
CHEMBL3883542 1 8.09
CHEMBL3884714 1 7.76
CHEMBL3884584 1 7.75
CHEMBL3885389 1 7.73
CHEMBL3883517 1 7.61
CHEMBL3884778 1 7.59
CHEMBL3885319 1 7.47
CHEMBL3883995 1 7.28
CHEMBL3885282 1 7.04
CHEMBL3885178 1 7.00
CHEMBL3884137 1 6.96
CHEMBL3884728 1 6.78
CHEMBL3884634 1 6.11
CHEMBL3884743 1 5.99
CHEMBL282199 1 5.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL56 8-OH-DPAT Ki = 2.65 nM 8.58
CHEMBL3884095 Ki = 2.99 nM 8.52
CHEMBL3883640 Ki = 6.96 nM 8.16
CHEMBL3883542 Ki = 8.21 nM 8.09
CHEMBL3884714 Ki = 17.4 nM 7.76
CHEMBL3884584 Ki = 17.8 nM 7.75
CHEMBL3885389 Ki = 18.6 nM 7.73
CHEMBL3883517 Ki = 24.7 nM 7.61
CHEMBL3884778 Ki = 25.9 nM 7.59
CHEMBL3885319 Ki = 33.5 nM 7.47
CHEMBL3883995 Ki = 52.1 nM 7.28
CHEMBL3885282 Ki = 90.3 nM 7.04
CHEMBL3885178 Ki = 100.0 nM 7.00
CHEMBL3884137 Ki = 111.0 nM 6.96
CHEMBL3884728 Ki = 167.0 nM 6.78
CHEMBL3884634 Ki = 772.0 nM 6.11
CHEMBL3884743 Ki = 1032.0 nM 5.99
CHEMBL282199 Ki = 9024.0 nM 5.04