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Assay Detail

CHEMBL3878910

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity against human adenosine A2A receptor expressed in CHO cells assessed as inhibition of CGS21680-stimulated cAMP accumulation measured after 150 mins in presence of [3H]cAMP by scintillation counting method
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2a (CHEMBL251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Pharmacological Characterization of 2-(2-Furanyl)thiazolo[5,4-d]pyrimidine-5,7-diamine Derivatives: New Highly Potent A2A Adenosine Receptor Inverse Agonists with Antinociceptive Activity.
Varano F, Catarzi D, Vincenzi F, Betti M, Falsini M, Ravani A, Borea PA, Colotta V, Varani K.
J Med Chem (2016) 59 : 10564 -10576
PubMed 27933962 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 8.31 10.29

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3958838 1 10.29
CHEMBL3904408 1 10.02
CHEMBL113142 ZM-241385 1 9.17
CHEMBL3935610 1 7.80
CHEMBL3950142 1 7.75
CHEMBL3908770 1 7.68
CHEMBL3932413 1 7.66
CHEMBL3934626 1 7.51
CHEMBL3931329 1 6.91

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3958838 IC50 = 0.051 nM 10.29
CHEMBL3904408 IC50 = 0.095 nM 10.02
CHEMBL113142 ZM-241385 IC50 = 0.678 nM 9.17
CHEMBL3935610 IC50 = 16.0 nM 7.80
CHEMBL3950142 IC50 = 18.0 nM 7.75
CHEMBL3908770 IC50 = 21.0 nM 7.68
CHEMBL3932413 IC50 = 22.0 nM 7.66
CHEMBL3934626 IC50 = 31.0 nM 7.51
CHEMBL3931329 IC50 = 123.0 nM 6.91