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Assay Detail

CHEMBL3879170

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MB064 probe binding to ABHD6 in mouse brain membrane proteome preincubated for 30 mins followed by MB064 probe addition measured after 20 mins by gel-based competitive activity based protein profiling assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Tissue Brain
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Monoacylglycerol lipase ABHD6 (CHEMBL5010)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Triazole Ureas Act as Diacylglycerol Lipase Inhibitors and Prevent Fasting-Induced Refeeding.
Deng H, Kooijman S, van den Nieuwendijk AM, Ogasawara D, van der Wel T, van Dalen F, Baggelaar MP, Janssen FJ, van den Berg RJ, den Dulk H, Cravatt BF, Overkleeft HS, Rensen PC, van der Stelt M.
J Med Chem (2017) 60 : 428 -440
PubMed 27992221 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.83 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3913807 1 7.30
CHEMBL3895863 1 7.10
CHEMBL3897587 1 6.50
CHEMBL3922787 1 6.40
CHEMBL3967137 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3913807 IC50 = 50.12 nM 7.30
CHEMBL3895863 IC50 = 79.43 nM 7.10
CHEMBL3897587 IC50 = 316.23 nM 6.50
CHEMBL3922787 IC50 = 398.11 nM 6.40
CHEMBL3967137 IC50 > 10000.0 nM -