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Assay Detail

CHEMBL3880189

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human S1P4 expressed in bla TANGO U2OS cells assessed as inhibition of cAMP formation preincubated for 4 hrs followed by FRET-based beta-lactamase fluorescent substrate addition measured after 2 hrs
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type U2OS
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sphingosine 1-phosphate receptor 4 (CHEMBL3230)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selective sphingosine 1 phosphate receptor modulators and methods of chiral synthesis
(2013)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 5.51 6.13

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3910269 1 6.13
CHEMBL3894716 1 5.92
CHEMBL3951270 1 5.79
CHEMBL3922179 1 5.49
CHEMBL3707247 OZANIMOD 4.0 1 5.38
CHEMBL3919445 1 5.26
CHEMBL3899384 1 5.10
CHEMBL3902160 1 5.04
CHEMBL3921214 1 -
CHEMBL3922998 1 -
CHEMBL3933952 1 -
CHEMBL3931243 1 -
CHEMBL3895230 1 -
CHEMBL3893172 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3910269 EC50 = 739.0 nM 6.13
CHEMBL3894716 EC50 = 1210.0 nM 5.92
CHEMBL3951270 EC50 = 1610.0 nM 5.79
CHEMBL3922179 EC50 = 3250.0 nM 5.49
CHEMBL3707247 OZANIMOD EC50 = 4192.0 nM 5.38
CHEMBL3919445 EC50 = 5500.0 nM 5.26
CHEMBL3899384 EC50 = 7866.0 nM 5.10
CHEMBL3902160 EC50 = 9034.0 nM 5.04
CHEMBL3921214 EC50 > 10000.0 nM -
CHEMBL3922998 EC50 > 10000.0 nM -
CHEMBL3933952 EC50 > 10000.0 nM -
CHEMBL3931243 EC50 > 10000.0 nM -
CHEMBL3895230 EC50 > 10000.0 nM -
CHEMBL3893172 EC50 > 10000.0 nM -