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Assay Detail

CHEMBL3880964

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length human AKT1 expressed in Sf9 cells assessed as reduction in substrate phosphorylation using biotin-ahx-ARKRERAYSFGHHA-amide substrate and [gamma-33P]ATP incubated for 40 mins by top count microplate scintillation counting method
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

RAC-alpha serine/threonine-protein kinase (CHEMBL4282)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibitors of AKT Activity
(2011)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 8.90 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2219422 AFURESERTIB 3.0 1 9.00
CHEMBL3953878 1 9.00
CHEMBL3137336 UPROSERTIB 2.0 1 8.70
CHEMBL3890070 1 -
CHEMBL3952025 1 -
CHEMBL3942525 1 -
CHEMBL3907254 1 -
CHEMBL3904492 1 -
CHEMBL3968297 1 -
CHEMBL3965875 1 -
CHEMBL3926382 1 -
CHEMBL3923631 1 -
CHEMBL3892820 1 -
CHEMBL3963487 1 -
CHEMBL3953883 1 -
CHEMBL3929383 1 -
CHEMBL3932335 1 -
CHEMBL3935057 1 -
CHEMBL3971992 1 -
CHEMBL3903718 1 -
CHEMBL3905729 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2219422 AFURESERTIB IC50 = 1.0 nM 9.00
CHEMBL3953878 IC50 = 1.0 nM 9.00
CHEMBL3137336 UPROSERTIB IC50 = 1.995 nM 8.70
CHEMBL3890070 IC50 <= 2.512 nM -
CHEMBL3952025 IC50 <= 2.512 nM -
CHEMBL3942525 IC50 <= 2.512 nM -
CHEMBL3907254 IC50 <= 2.512 nM -
CHEMBL3904492 IC50 <= 2.512 nM -
CHEMBL3968297 IC50 <= 2.512 nM -
CHEMBL3965875 IC50 <= 2.512 nM -
CHEMBL3926382 IC50 <= 2.512 nM -
CHEMBL3923631 IC50 <= 2.512 nM -
CHEMBL3892820 IC50 <= 2.512 nM -
CHEMBL3963487 IC50 <= 2.512 nM -
CHEMBL3953883 IC50 <= 2.512 nM -
CHEMBL3929383 IC50 <= 2.512 nM -
CHEMBL3932335 IC50 <= 2.512 nM -
CHEMBL3935057 IC50 <= 2.512 nM -
CHEMBL3971992 IC50 <= 2.512 nM -
CHEMBL3903718 IC50 <= 2.512 nM -
CHEMBL3905729 IC50 <= 2.512 nM -