Assay Detail
Binding
CHEMBL3887256
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Kinase Assay: Met (h) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 250 μM KKKSPGEYVNIEFG, 10 mM MgAcetate and [γ-33P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 3% phosphoric acid solution. 10 μL of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Substituted quinoline compounds and methods of use
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 7 | 7.86 | 8.15 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4107720 | — | — | 1 | 8.15 |
| CHEMBL3919169 | — | — | 1 | 8.00 |
| CHEMBL3920222 | — | — | 1 | 7.89 |
| CHEMBL3910664 | — | — | 1 | 7.89 |
| CHEMBL4115455 | — | — | 1 | 7.82 |
| CHEMBL3984441 | NINGETINIB | 2.0 | 1 | 7.72 |
| CHEMBL3963800 | — | — | 1 | 7.57 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4107720 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL3919169 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL3920222 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL3910664 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL4115455 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL3984441 | NINGETINIB | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL3963800 | — | IC50 | = | 27.0 | nM | 7.57 |