Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3887285

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
CB1 Receptor Binding Protocol: 8 ug of membranes (20 ul of 1:8 dilution in incubation buffer) was incubated with 5 ul of drug solution (10E-4M to 10E-12M) and 5ul of 5.4 nM [3H]CP55,940 in a total volume of 200 ul for 90 mins at 30 C. Non-specific binding was determined using 10 uM WIN55,212-2 (Ki=4.4 nM). The membranes were filtered and the filters washed 7x with 0.2 ml ice-cold incubation buffer and allowed to air dry under vacuum.
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Cannabinoid receptor 1 (CHEMBL218)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tri-aryl/heteroaromatic cannabinoids and use thereof
(2015)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 16 7.31 8.63

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3959623 1 8.63
CHEMBL3923170 1 8.43
CHEMBL3928596 1 7.76
CHEMBL460545 1 7.57
CHEMBL3915185 1 7.38
CHEMBL3922001 1 7.33
CHEMBL525067 1 7.03
CHEMBL3950898 1 6.70
CHEMBL1081624 1 6.30
CHEMBL3907723 1 6.01
CHEMBL460544 1 -
CHEMBL1076685 1 -
CHEMBL3978261 1 -
CHEMBL3967280 1 -
CHEMBL460754 1 -
CHEMBL3937621 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3959623 Ki = 2.33 nM 8.63
CHEMBL3923170 Ki = 3.7 nM 8.43
CHEMBL3928596 Ki = 17.18 nM 7.76
CHEMBL460545 Ki = 27.0 nM 7.57
CHEMBL3915185 Ki = 41.96 nM 7.38
CHEMBL3922001 Ki = 46.71 nM 7.33
CHEMBL525067 Ki = 93.66 nM 7.03
CHEMBL3950898 Ki = 199.0 nM 6.70
CHEMBL1081624 Ki = 503.0 nM 6.30
CHEMBL3907723 Ki = 983.3 nM 6.01
CHEMBL460544 Ki > 1000.0 nM -
CHEMBL1076685 Ki > 1000.0 nM -
CHEMBL3978261 Ki > 1000.0 nM -
CHEMBL3967280 Ki > 1000.0 nM -
CHEMBL460754 Ki > 1000.0 nM -
CHEMBL3937621 Ki > 1000.0 nM -