Assay Detail
Binding
CHEMBL3888870
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Enzyme Inhibition Assay: The enzyme reaction (total volume 10 μl) was carried out in black 384-well low volume plates containing full length MPS1 (12.5 nM or 3 nM), fluorescent labelled peptide [known as H236, which has the sequence: 5FAM-DHTGFLTEYVATR-CONH2] (5 μM), ATP(10 μM), either DMSO (1% v/v) or the test compound (in the range 0.25 nM-100 μM in 1% DMSO) and assay buffer (50 mM HEPES (pH 7.0), 0.02% NaN3, 0.01% BSA, 0.1 mM Orthovandate, 10 μM MgCl2, 1 μM DTT, Roche protease inhibitor). The reaction was carried out for 60 min at room temperature and stopped by the addition of buffer (10 μl) containing 20 mM EDTA, 0.05% (v/v) Brij-35, in 0.1M HEPES-buffered saline (Free acid, Sigma, UK). The plate was read on a Caliper EZ reader II (Caliper Life Sciences).
205
Total Activities
204
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Inhibitor compounds
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 205 | 7.94 | 9.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3928170 | — | — | 1 | 9.00 |
| CHEMBL3913772 | — | — | 1 | 9.00 |
| CHEMBL3919267 | — | — | 1 | 8.70 |
| CHEMBL3943432 | — | — | 1 | 8.70 |
| CHEMBL3940708 | — | — | 1 | 8.70 |
| CHEMBL3946383 | — | — | 1 | 8.70 |
| CHEMBL3910642 | — | — | 1 | 8.70 |
| CHEMBL3932996 | — | — | 1 | 8.70 |
| CHEMBL3890427 | — | — | 1 | 8.70 |
| CHEMBL3925302 | — | — | 1 | 8.70 |
| CHEMBL3897889 | — | — | 1 | 8.70 |
| CHEMBL3919425 | — | — | 1 | 8.70 |
| CHEMBL3906901 | — | — | 1 | 8.70 |
| CHEMBL3911468 | — | — | 1 | 8.70 |
| CHEMBL3962472 | — | — | 1 | 8.70 |
| CHEMBL3941672 | — | — | 1 | 8.52 |
| CHEMBL3952394 | — | — | 1 | 8.52 |
| CHEMBL3908752 | — | — | 1 | 8.52 |
| CHEMBL3944189 | — | — | 1 | 8.52 |
| CHEMBL3907282 | — | — | 1 | 8.52 |
| CHEMBL3901324 | — | — | 1 | 8.52 |
| CHEMBL3970871 | — | — | 1 | 8.52 |
| CHEMBL3915042 | — | — | 1 | 8.52 |
| CHEMBL3941982 | — | — | 1 | 8.52 |
| CHEMBL3893532 | — | — | 1 | 8.52 |
| CHEMBL3907465 | — | — | 1 | 8.52 |
| CHEMBL3939747 | — | — | 1 | 8.52 |
| CHEMBL3890400 | — | — | 1 | 8.52 |
| CHEMBL3976598 | — | — | 1 | 8.52 |
| CHEMBL3930923 | — | — | 1 | 8.52 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3913772 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL3928170 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL3911468 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3962472 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3925302 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3906901 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3890427 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3932996 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3919267 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3946383 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3940708 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3919425 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3897889 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3910642 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3943432 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3941982 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3956274 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3941672 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3901423 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3939747 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3912889 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3930923 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3976598 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3925962 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3890400 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3952394 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3907428 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3900773 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3967187 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3907465 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3929386 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3893532 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3944189 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3908752 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3920481 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3907282 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3901324 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3966905 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3924132 | BOS-172722 | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3917528 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3970871 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3915042 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3951218 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3972481 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3958301 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3956968 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3955250 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3943831 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3986071 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3934920 | — | IC50 | = | 4.0 | nM | 8.40 |