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Assay Detail

CHEMBL3889079

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Binding
In Vitro Assay: Plasma kallikrein determinations were made in 0.1 M sodium phosphate buffer at a pH of 7.5 containing 0.1-0.2 M sodium chloride and 0.5% PEG 8000. Determinations were made using purified human plasma kallikrein (Enzyme Research Laboratories) at a final assay concentration of 200 pM and the synthetic substrate S-2302 (H-(D)-Pro-Phe-Arg-pNA; CHROMOGENIX®) at a concentration of 0.00008-0.0004 M.
11
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Plasma
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Plasma kallikrein (CHEMBL2000)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pyrimidinones as factor XIa inhibitors
(2016)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 7.62 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4108841 1 8.00
CHEMBL4113006 2 7.77
CHEMBL4111839 2 7.66
CHEMBL4107758 2 7.62
CHEMBL4112929 MILVEXIAN 3.0 1 7.55
CHEMBL4115014 1 7.48
CHEMBL4107075 1 7.46
CHEMBL4107761 1 7.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4108841 Ki = 10.0 nM 8.00
CHEMBL4113006 Ki = 17.0 nM 7.77
CHEMBL4113006 Ki = 19.0 nM 7.72
CHEMBL4111839 Ki = 22.0 nM 7.66
CHEMBL4111839 Ki = 23.0 nM 7.64
CHEMBL4107758 Ki = 24.0 nM 7.62
CHEMBL4112929 MILVEXIAN Ki = 28.0 nM 7.55
CHEMBL4107758 Ki = 32.0 nM 7.50
CHEMBL4115014 Ki = 33.0 nM 7.48
CHEMBL4107075 Ki = 35.0 nM 7.46
CHEMBL4107761 Ki = 37.0 nM 7.43