Assay Detail
Binding
CHEMBL3889157
Review assay metadata, readout intent, target linkage, and publication context from the same page.
ELISA Assay: CVF-Bb complex prepared from purified cobra venom factor (1 μM), recombinant human complement factor B (expressed in drosophila cells and purified using standard methods) and human complement factor D (expressed in E. Coli, refolded and purified using standard methods). CVF-Bb complex at 3 nM concentration was incubated with test compound at various concentrations for 1 hour at room temperature in PBS pH 7.4 containing 10 mM MgCl2 and 0.05% (w/v) CHAPS. Human complement C3 substrate purified from plasma was added to a final concentration of 1 μM. After 1 hour incubation at room temperature, the enzyme reaction was stopped by addition of a cocktail of concentrated pan-protease inhibitors. The product of the reaction, C3a, was quantified by means of an enzyme-linked-immunosorbent assay.
131
Total Activities
130
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Tissue | Plasma |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Complement pathway modulators and uses thereof
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 131 | 7.02 | 9.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4106993 | — | — | 1 | 9.00 |
| CHEMBL4114217 | — | — | 1 | 9.00 |
| CHEMBL4107243 | — | — | 1 | 8.52 |
| CHEMBL4113469 | — | — | 1 | 8.52 |
| CHEMBL4108959 | — | — | 1 | 8.40 |
| CHEMBL4111681 | — | — | 1 | 8.40 |
| CHEMBL4114574 | — | — | 1 | 8.30 |
| CHEMBL4107774 | — | — | 1 | 8.22 |
| CHEMBL3928150 | — | — | 1 | 8.22 |
| CHEMBL4114991 | — | — | 1 | 8.22 |
| CHEMBL4114221 | — | — | 1 | 8.22 |
| CHEMBL4107659 | — | — | 1 | 8.22 |
| CHEMBL4109971 | — | — | 1 | 8.22 |
| CHEMBL4115570 | — | — | 1 | 8.22 |
| CHEMBL4110511 | — | — | 1 | 8.15 |
| CHEMBL3943926 | — | — | 1 | 8.15 |
| CHEMBL4111301 | — | — | 1 | 8.10 |
| CHEMBL3957678 | — | — | 1 | 8.05 |
| CHEMBL4112178 | — | — | 1 | 8.00 |
| CHEMBL3981421 | — | — | 1 | 8.00 |
| CHEMBL4114531 | — | — | 1 | 8.00 |
| CHEMBL4113781 | — | — | 1 | 8.00 |
| CHEMBL3952833 | — | — | 1 | 7.96 |
| CHEMBL3905588 | — | — | 1 | 7.96 |
| CHEMBL4113062 | — | — | 1 | 7.96 |
| CHEMBL4112415 | — | — | 1 | 7.92 |
| CHEMBL4113623 | — | — | 1 | 7.89 |
| CHEMBL4113997 | — | — | 1 | 7.85 |
| CHEMBL3923445 | — | — | 1 | 7.82 |
| CHEMBL4108762 | — | — | 1 | 7.82 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4114217 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL4106993 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL4107243 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL4113469 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL4111681 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL4108959 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL4114574 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL4115570 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL4109971 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL4107774 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL4107659 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL4114221 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL4114991 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3928150 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL4110511 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL3943926 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL4111301 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL3957678 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL4113781 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL4114531 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL3981421 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL4112178 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL3952833 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL4113062 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL3905588 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL4112415 | — | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL4113623 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL4113997 | — | IC50 | = | 14.0 | nM | 7.85 |
| CHEMBL4108762 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL3923445 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL3975396 | — | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL3898919 | — | IC50 | = | 17.0 | nM | 7.77 |
| CHEMBL3929496 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL4107253 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL3890117 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL3978495 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL3953356 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL3968840 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL4107492 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL3973437 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL4109043 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL4110121 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL4109775 | — | IC50 | = | 40.0 | nM | 7.40 |
| CHEMBL3957065 | — | IC50 | = | 40.0 | nM | 7.40 |
| CHEMBL4111694 | — | IC50 | = | 40.0 | nM | 7.40 |
| CHEMBL4115080 | — | IC50 | = | 40.0 | nM | 7.40 |
| CHEMBL4115621 | — | IC50 | = | 40.0 | nM | 7.40 |
| CHEMBL4110860 | — | IC50 | = | 40.0 | nM | 7.40 |
| CHEMBL4113394 | — | IC50 | = | 50.0 | nM | 7.30 |
| CHEMBL4108466 | — | IC50 | = | 50.0 | nM | 7.30 |