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Assay Detail

CHEMBL3889231

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Cholesteryl Ester Transfer Assay: As a protein source for cholesteryl ester transfer, plasma from healthy persons was used, and as a cholesteryl ester receptor, LDL from healthy persons was used. Samples were treated with each test compound to final concentrations of 16, 80, 400, 2000 and 10000 nM and analyzed in duplicate. For the cholesteryl ester transfer test, 20 μl of plasma, 50 μl of LDL (0.25 mg/ml) and 50 μl of rHDL-agarose (0.25 mg/ml) were added, and a solution containing a test compound was added, followed by reaction at 37° C. Then, centrifugation was performed at 4° C. for 3 minutes to stop the reaction, and 150 μl of the supernatant was taken and transferred to a 96-well plate for radioactivity measurement, and the radioactivity of the plate was measured with a beta-ray detector.
41
Total Activities
41
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Plasma
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Cholesteryl ester transfer protein (CHEMBL3572)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Biaryl- or heterocyclic biaryl-substituted cyclohexene derivative compounds as CETP inhibitors
(2016)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 41 8.04 8.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3906320 1 8.82
CHEMBL3914103 1 8.80
CHEMBL3965631 1 8.72
CHEMBL3955558 1 8.72
CHEMBL3982930 1 8.60
CHEMBL3972983 1 8.41
CHEMBL3953367 1 8.30
CHEMBL3948773 1 8.28
CHEMBL3986115 1 8.27
CHEMBL3927226 1 8.24
CHEMBL3898351 1 8.23
CHEMBL3987174 1 8.19
CHEMBL3941193 1 8.18
CHEMBL3915159 1 8.16
CHEMBL3926422 1 8.15
CHEMBL3932190 1 8.14
CHEMBL3912017 1 8.12
CHEMBL3928848 1 8.12
CHEMBL3949935 1 8.08
CHEMBL3897170 1 8.05
CHEMBL3891904 1 8.04
CHEMBL3920961 1 8.02
CHEMBL3926760 1 7.97
CHEMBL3942098 1 7.94
CHEMBL3919350 1 7.94
CHEMBL3954404 1 7.92
CHEMBL3897353 1 7.91
CHEMBL3980987 1 7.90
CHEMBL3978985 1 7.89
CHEMBL3943442 1 7.84

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3906320 IC50 = 1.5 nM 8.82
CHEMBL3914103 IC50 = 1.6 nM 8.80
CHEMBL3955558 IC50 = 1.9 nM 8.72
CHEMBL3965631 IC50 = 1.9 nM 8.72
CHEMBL3982930 IC50 = 2.5 nM 8.60
CHEMBL3972983 IC50 = 3.9 nM 8.41
CHEMBL3953367 IC50 = 5.0 nM 8.30
CHEMBL3948773 IC50 = 5.3 nM 8.28
CHEMBL3986115 IC50 = 5.4 nM 8.27
CHEMBL3927226 IC50 = 5.8 nM 8.24
CHEMBL3898351 IC50 = 5.9 nM 8.23
CHEMBL3987174 IC50 = 6.4 nM 8.19
CHEMBL3941193 IC50 = 6.6 nM 8.18
CHEMBL3915159 IC50 = 6.9 nM 8.16
CHEMBL3926422 IC50 = 7.1 nM 8.15
CHEMBL3932190 IC50 = 7.3 nM 8.14
CHEMBL3912017 IC50 = 7.5 nM 8.12
CHEMBL3928848 IC50 = 7.6 nM 8.12
CHEMBL3949935 IC50 = 8.4 nM 8.08
CHEMBL3897170 IC50 = 8.9 nM 8.05
CHEMBL3891904 IC50 = 9.1 nM 8.04
CHEMBL3920961 IC50 = 9.6 nM 8.02
CHEMBL3926760 IC50 = 10.7 nM 7.97
CHEMBL3942098 IC50 = 11.6 nM 7.94
CHEMBL3919350 IC50 = 11.4 nM 7.94
CHEMBL3954404 IC50 = 11.9 nM 7.92
CHEMBL3897353 IC50 = 12.3 nM 7.91
CHEMBL3980987 IC50 = 12.6 nM 7.90
CHEMBL3978985 IC50 = 13.0 nM 7.89
CHEMBL3943442 IC50 = 14.6 nM 7.84
CHEMBL3971977 IC50 = 15.0 nM 7.82
CHEMBL3970749 IC50 = 15.9 nM 7.80
CHEMBL3907248 IC50 = 15.9 nM 7.80
CHEMBL3934300 IC50 = 17.9 nM 7.75
CHEMBL3910458 IC50 = 20.3 nM 7.69
CHEMBL3957430 IC50 = 23.2 nM 7.63
CHEMBL3924471 IC50 = 24.3 nM 7.61
CHEMBL3919949 IC50 = 27.0 nM 7.57
CHEMBL3951447 IC50 = 28.4 nM 7.55
CHEMBL3890674 IC50 = 50.2 nM 7.30
CHEMBL3897306 IC50 = 56.6 nM 7.25